invivo imaginganddetectionofnitroreductaseinzebra ? shbyanewnear-infrared ? uorescenceoff – onprobe
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资料摘要:
Short communication
in vivo imaging and detection of nitroreductase in zebrafish by a new
near-infraredfluorescence off–on probe
Zhao Li
a
, Xinyuan He
a
, Zhe Wang
a
, Ronghua Yang
b
, Wen Shi
a
, Huimin Ma
a,n
a
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry,
Chinese Academy of Sciences, Beijing 100190, China
b
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China
article info
Article history:
Received 9 June 2014
Received in revised form
8 July 2014
Accepted 9 July 2014
Available online 16 July 2014
Keywords:
Nitroreductase
Near-infraredfluorescence probe
in vivo imaging
Zebrafish
abstract
A new near-infraredfluorescence off–on probe is developed and applied tofluorescence imaging of
nitroreductase in zebrafish in vivo. The probe is readily prepared by connecting 4-nitrobenzene as a
quenching and recognizing moiety to a stable hemicyanine skeleton that can be formed via the
decomposition of IR 780. Thefluorescence off–on response of the probe to nitroreductase is based on
the enzyme-catalyzed reduction of the 4-nitrobenzene moiety, followed by the 1,6-rearrangementelimination and the fluorophore release. Compared with the existing nitroreductase probes, the
proposed probe exhibits superior analytical performance such as near-infraredfluorescence emission
over 700 nm as well as high selectivity and sensitivity, with a detection limit of 14 ng/mL. More
importantly, the probe has been successfully applied to visualize the distribution of nitroreductase in
living zebrafish in vivo, revealing that nitroreductase might mainly exist in zebrafish yolk sac. The
superior properties of the probe make it of great potential use in other biosystems andin vivo studies.
&2014 Elsevier B.V. All rights reserved.