人抗载脂蛋白抗体A1试剂盒，(ApoA1）Elisa Kit

产品名称：人抗载脂蛋白抗体A1试剂盒，(ApoA1）Elisa Kit。

产品性状：液体

检测标本：血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等

产品用途：科研实验专用

产品规格：96T/48T

产品存储：4°保存

本试剂盒采用双抗体夹心ELISA法。用抗体包被于酶标板上，实验时标本或标准品会与包被抗体结合，游离的成分被洗去。依次加入生物素化抗体和辣根过氧化物酶标记的亲和素。抗体与结合在包被抗体上结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物，TMB在辣根过氧化物酶的催化下现蓝色，加终止液后变黄。用酶标仪在450nm波长处测OD值，浓度与OD450值之间呈正比，通过绘制标准曲线求出标本浓度。

人抗载脂蛋白抗体A1试剂盒，(ApoA1）Elisa Kit，注意事项：

1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。

2. 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。

3. 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。

5. 封板膜只限一次性使用，以避免交叉污染。

6. 底物请避光保存。

7. 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.

8. 所有样品，洗涤液和各种废弃物都应按传染物处理。

9. 本试剂不同批号组分不得混用。

10.如与英文说明书有异，以英文说明书为准。

人抗载脂蛋白抗体A1试剂盒，(ApoA1）Elisa Kit，ELISA的基本原理有三条：

1、抗原或抗体能以物理性地吸附于固相载体表面，可能是蛋白和聚苯乙烯表面间的疏水性部分相互吸附，并保持其免疫学活性；

2、抗原或抗体可通过共价键与酶连接形成酶结合物，而此种酶结合物仍能保持其免疫学和酶学活性；

3、酶结合物与相应抗原或抗体结合后，可根据加入底物的颜色反应来判定是否有免疫反应的存在，而且颜色反应的深浅是与标本中相应抗原或抗体的量成正比例的，因此，可以按底物显色的程度显示试验结果。

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E-10447 人抗Sa抗体试剂盒，(anti-Sa-Ab）Elisa Kit Human anti-Sa-antibody ELISA Kit
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