人脑红蛋白(NGB)ELISA试剂盒原理
辣根过氧化物酶 (horse radish peroxidase,简称HRP,EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶,早在20世纪30年代就有人着手从辣根中分离此酶,以后又制备出结晶。本实验是以辣根为原料,经过水的抽提,硫酸铵和丙酮分级分离,再经锌离子纯化,透析除盐,冰冻干燥,便可得到高纯度的辣根过氧化物酶。
人脑红蛋白(NGB)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质,HRP广泛分布于植物界,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,Mr在40000左右,等电点7.2。溶于水,溶解度为5%(W/V),溶液呈棕红色,透明。人脑红蛋白(NGB)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异,但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液,而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下,HRP几周内稳定,加热到63℃,在15min内稳定。人脑红蛋白(NGB)ELISA试剂盒氧化还原电势很低,pH6.08时,E 0 =-0.2070V,pH为7.71时,E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b
酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否,因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体,它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体,其次要有良好的制备方法。目前,高质量的酶(如辣根过氧化物酶,简称HRP)国内已有商品供应。人脑红蛋白(NGB)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上,宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。
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XY-EL-H1267c 人肺表面活性物质相关蛋白B(SPB)ELISA试剂盒 Human SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
XY-EL-H1268c 人肺表面活性物质相关蛋白C(SPC)ELISA试剂盒 Human SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
XY-EL-H1269c 人肺表面活性物质相关蛋白D(SPD)ELISA试剂盒 Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
XY-EL-H1270c 人肺部活化调节趋化因子(PARC/CCL18)ELISA试剂盒 Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit
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XY-EL-H1274c 人信号素3A(SEMA3A)ELISA试剂盒 Human SEMA3A (Semaphorin 3A) ELISA Kit
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XY-EL-H1279c 人血清淀粉样蛋白P(SAP)ELISA试剂盒 Human SAP (Serum Amyloid P Component) ELISA Kit
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XY-EL-H1283c 人G蛋白偶联受体120(GPR120)ELISA试剂盒 Human GPR120 (G Protein Coupled Receptor 120) ELISA Kit
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XY-EL-H1292c 人S100钙结合蛋白A11(S100A11)ELISA试剂盒 Human S100A11 (S100 Calcium Binding Protein A11) ELISA Kit
XY-EL-H1293c 人S100钙结合蛋白A12(S100A12 )ELISA试剂盒 Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
XY-EL-H1294c 人S100钙结合蛋白A13(S100A13 )ELISA试剂盒 Human S100A13 (S100 Calcium Binding Protein A13) ELISA Kit
XY-EL-H1295c 人S100钙结合蛋白A6(S100A6)ELISA试剂盒 Human S100A6 (S100 Calcium Binding Protein A6) ELISA Kit
XY-EL-H1296c 人S100钙结合蛋白A7 (S100A7)ELISA试剂盒 Human S100A7 (S100 Calcium Binding Protein A7) ELISA Kit
XY-EL-H1297c 人S100钙结合蛋白B (S100B)ELISA试剂盒 Human S100B (S100 Calcium Binding Protein B) ELISA Kit
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