凝血因子II(F2)ELISA检测试剂盒
中文简称:凝血因子II(F2)ELISA检测试剂盒
适应物种:Human
标记物:详见说明书
检测限:详见说明书
数量:大量
样本:详见说明书
规格:96T/Kit
凝血因子II(F2)ELISA检测试剂盒实验误差减小分为以下几种:
1.做实验时少说话,防止唾液掉进酶标条中。
2.加入一抗二抗需要放入37°。
3.如果同时做多个板子,多个板子别罗一起;尽量少用排枪。
4.加样时,由低浓度向高浓度加,这样减少跳孔的几率。
5.勤换枪头。
6.移液器的使用,加样(抗体)等需要准确定量的试剂时不使用排枪,经验证明排枪很不准确,极易跳孔,不要图便宜买低档的tips 因为ELISA不但会放大被检测的目的蛋白,也会放大非特异结合的杂质蛋白。
倍比稀释样品时,稀释倍数最好要小于10。
7.所有的装跟凝血因子II(F2)ELISA检测试剂盒相关试剂的容器,玻璃制品的要干烤过或者使用一次性的树脂容器。
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