人蛋白质二硫键异构酶(PDI)ELISA Kit
产品类型:
ELISA Kit
产品名称:
人蛋白质二硫键异构酶(PDI)ELISA Kit
英文名称:
Human protein disulfide isomerase,PDI ELISA Kit
货号:
人蛋白质二硫键异构酶(PDI)ELISA Kit
别名:
DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, collagen prolyl 4-hydroxylase beta|glutathione-insulin transhydrogenase|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4
规格:
96T
中文价格:
人蛋白质二硫键异构酶(PDI)ELISA Kit
种属:
Human
待测物名称:
prolyl 4-hydroxylase, beta polypeptide
缩写:
P4HB
检测范围:
0.312 ng/ml-20 ng/ml
灵敏度:
0.078 ng/ml
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is人蛋白质二硫键异构酶(PDI)ELISA Kit bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.
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