大鼠亮氨酰氨基肽酶(LAP)ELISA Kit
产品类型:
ELISA Kit
产品名称:
大鼠亮氨酰氨基肽酶(LAP)ELISA Kit
英文名称:
Rat Leucine aminopepridase,LAP ELISA Kit
大鼠亮氨酰氨基肽酶(LAP)ELISA Kit
别名:
LAP, LAPEP, PEPS, peptidase S
规格:
96T
大鼠亮氨酰氨基肽酶(LAP)ELISA Kit
种属:
Rat
待测物名称:
leucine aminopeptidase 3
缩写:
LAP3
检测范围:
6.25 U/ml-400 U/ml
灵敏度:
1.56 U/ml
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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