白介素1受体关联激酶1(IRAK1)检测试剂盒

白介素1受体关联激酶1(IRAK1)检测试剂盒

适用生物     Homo sapiens (Human，人)    

白介素1受体关联激酶1(IRAK1)检测试剂盒    

检测范围     0.313-20ng/mL     灵敏度     0.116ng/mL    

样本类型     Tissue homogenates, cell lysates and other biological fluids.    

实验时长     4.5h     实验方法     双抗夹心法    白介素1受体关联激酶1(IRAK1)检测试剂盒

规格     96T    

ELISA Kit for Interleukin 1 Receptor Associated Kinase 1 (IRAK1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species     Homo sapiens (Human)    

白介素1受体关联激酶1(IRAK1)检测试剂盒  

Sample type     Tissue homogenates, cell lysates and other biological fluids.    

Format     96-well strip plate    

Assay length     4.5 hours    

Detection range     0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL    

Sensitivity     The minimum detectable dose of this kit is typically less than 0.116ng/mL.    

Specificity

This assay has high sensitivity 白介素1受体关联激酶1(IRAK1)检测试剂盒and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 1 (IRAK1).
No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 1 (IRAK1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 1 (IRAK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated 白介素1受体关联激酶1(IRAK1)检测试剂盒Kinase 1 (IRAK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, 白介素1受体关联激酶1(IRAK1)检测试剂盒air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents     Quantity     Reagents     Quantity    

Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    

Standard     2     Standard Diluent     1×20mL    

Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    

Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    

TMB Substrate     1×9mL     Stop Solution     1×6mL    

Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    

Assay procedure summary

1. Prepare all reagents, 白介素1受体关联激酶1(IRAK1)检测试剂盒samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediately.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been 白介素1受体关联激酶1(IRAK1)检测试剂盒pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 1 (IRAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 1 (IRAK1) in the samples 白介素1受体关联激酶1(IRAK1)检测试剂盒is then determined by comparing the O.D. of the samples to the standard curve.