磷酸化蛋白激酶C α/β2抗体

英文名称    Phospho-PKC alpha/beta II (Thr638/641)    

中文名称    磷酸化蛋白激酶C α/β2抗体    

别    名    PKC(Phospho-Thr641); PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKC alpha; PKCA; PRKACA; PRKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type; KPCB_HUMAN.    

磷酸化蛋白激酶C α/β2抗体      

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  免疫学  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Cow, Horse,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    77kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthesised phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化蛋白激酶C α/β2抗体  

PubMed    PubMed    

产品介绍    background:
Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

Function:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).

Subunit:
Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1 (By similarity). Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. Interacts with PICK1 (via PDZ domain). Interacts with TRIM41.

Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein (Probable). Nucleus.

Post-translational modifications:
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.

Gene ID:
5578

Database links:

Entrez Gene: 5578 Human

Entrez Gene: 18750 Mouse

Entrez Gene: 24680 Rat

Omim: 176960 Human

SwissProt: P17252 Human

SwissProt: P20444 Mouse

SwissProt: P05696 Rat

Unigene: 531704 Human

Unigene: 708867 Human

Unigene: 222178 Mouse

Unigene: 207908 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化蛋白激酶C α/β2抗体

hz-4818R CD45/B220  白细胞共同抗原抗体

hz-4819R CD45/B220  白细胞共同抗原抗体

hz-4820R CD45/B220  白细胞共同抗原抗体

hz-4821R AFP  甲胎蛋白AFP抗体

hz-4826R CD83  CD83抗体

hz-4834R CD73/NT5  CD73抗体

hz-4835R MAL/MPV17  T淋巴细胞成熟相关蛋白抗体

hz-4836R CXC-R2  CXCR2/CD182

hz-4838R HE4  附睾分泌蛋白4抗体

hz-4839R nonstructural protein 1/NS1  A型流感病毒-NS1抗体（神经氨酸酶1）

hz-4846R Factor VII  凝血因子7抗体

hz-4847R phospho-ITGB4(Tyr1530)  磷酸化整合素β4抗体

hz-4848R phospho-RUNX3(Ser338)  磷酸化Runx3抗体

hz-4849R GLUT2  葡萄糖转运蛋白2

hz-4850R Collagen II/Chondrocalcin  Ⅱ型胶原α1蛋白/软骨钙素抗体

hz-4851R Collagen II/Chondrocalcin  Ⅱ型胶原α1蛋白/软骨钙素抗体

hz-4852R PIM3  丝氨酸/苏氨酸蛋白激酶PIM3抗体

hz-4856R Hepatitis C Virus NS5a  丙型肝炎病毒NS5A抗体

hz-4857R Hepatitis C Virus NS4B  丙型肝炎病毒NS4B抗体

hz-4858R Hepatitis C Virus RNA-directed RNA polymerase  丙型肝炎病毒NS4B（65kDa）抗体

hz-4862R SORBS2/ArgBP2  精氨酸结合蛋白2抗体

hz-4865R BNP  脑钠素/利钠肽抗体

hz-4867R p70 S6 kinase beta  核糖体蛋白S6激酶1抗体

hz-4869R CD20  CD20抗体

hz-4871R Complhzent C3 beta chain  补体C3b抗体

hz-4872R C3a/C3a anaphylatoxin  补体C3a过敏毒素抗体

hz-4873R Complhzent C3b alpha' chain  补体C3b-α链抗体

hz-4884R CD33/Siglec-3  CD33抗体

hz-4886R TRhz1  髓系细胞触发受体1抗体

hz-4890R CD24  CD24抗体

hz-4891R CD24  CD24抗体

hz-4892R APOE/Apo E2  载脂蛋白E抗体

hz-4895R PIRH2  泛素连接酶抗体

hz-4902R Calponin 1/2/3  钙结合蛋白Calponin抗体

hz-4905R SORBS2/ArgBP2  精氨酸结合蛋白2抗体

hz-4906R SLC40A1/FPN1  细胞膜铁转运蛋白FP1抗体

hz-4907R TGF beta 3  转移生长因子β3（TGFβ3）抗体

hz-4908R TGF beta 1/LAP  转化生长因子β（TGFβ）抗体

hz-4909R TGF beta 2  转化生长因子β2（TGFβ2）抗体

hz-4910R MCSF/M-CSF  巨噬细胞克隆刺激因子抗体

hz-4911R Reg3 beta  胰岛β细胞生长因子Reg IIIα 抗体

hz-4912R Reg3 gamma  胰岛β细胞生长因子Reg IIIγ抗体

hz-4913R ENPP1  核苷酸内焦磷酸酶/磷酸二酯酶1抗体

hz-4915R ACCN1/BNaC1/ASIC2  脑钠通道蛋白1抗体