英文名称 Piwil2
中文名称 piwi样2蛋白抗体
别 名 HILI; Hiwi like; MILI; Miwi like; Piwi like 2; Piwi-like 2; Piwil 2; Piwil-2; Piwil2; Cancer/testis antigen 80; CT80; PIWL2_HUMAN.
piwi样2蛋白抗体
说 明 书 0.1ml 0.2ml
研究领域 肿瘤 细胞生物 免疫学 信号转导 转录调节因子
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 107kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Piwil2/Mili
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
piwi样2蛋白抗体
PubMed PubMed
产品介绍 background:
HILI is a 973 amino acid protein encoded by the human gene PIWIL2. HILI belongs to the argonaute family and contains one PAZ domain and one PIWI domain. HILI is a cytoplasmic protein that is expressed in adult testis and in most tumors. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. Expression of HILI can modulate expression of genes involved in stem cell proliferation (such as PDGFR-b), in energy metabolism (such as Glut1), in cell-cell interaction (such as Integrin a6, GJA7, THY-1 and CD9), and in germ cell differentiation (such as STRA8). It may also play a role as a regulatory factor of Stat3/Bcl-xS/L/CCND1 pathway. Repression of HILI can inhibit tumor cell growth. HILI acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Function:
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Subunit:
Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1.
Subcellular Location:
Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
Tissue Specificity:
Expressed in adult testis and in most tumors.
Post-translational modifications:
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
Similarity:
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Database links:
Entrez Gene: 55124 Human
Entrez Gene: 57746 Mouse
Entrez Gene: 306011 Rat
Omim: 610312 Human
SwissProt: Q8TC59 Human
SwissProt: Q8CDG1 Mouse
Unigene: 614809 Human
Unigene: 85253 Mouse
Unigene: 144968 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
piwi样2蛋白抗体
hz-3278R Phospho-MKP1 (Ser359) 磷酸化丝裂原活化蛋白激酶磷酸酶-1抗体
hz-3279R Phospho-MKP1 (Ser296 + Ser318) 磷酸化丝裂原活化蛋白激酶磷酸酶-1抗体
hz-1852R MKP-2/DUSP4 丝裂原活化蛋白激酶磷酸酶-2抗体
hz-0122R ER-α 雌激素受体α抗体
hzm-1591M ER/PR/c-erbB-2 Kit ER/PR/c-erbB-2检测试剂盒（鼠单抗）
hz-0894R Phospho-Estrogen Receptor alpha (Ser167) 磷酸化雌激素受体ER alpha 抗体
hz-1643R Phospho-Estrogen Receptor alpha (Tyr537) 磷酸化雌激素受体α抗体
hz-3130R Phospho-Estrogen Receptor alpha (Ser118) 磷酸化雌激素受体α抗体
hz-3131R Phospho-Estrogen Receptor alpha (Ser104/106) 磷酸化雌激素受体α抗体
hz-5338R Phospho-Estrogen Receptor alpha (Ser305) 磷酸化雌激素受体α抗体
hz-0254R ER-Alpha 雌激素受体α抗体（用于western blot）
hz-5895R WASF1/WAVE 1 WAVE1蛋白抗体
hz-6243R WASF2 WASF2抗体
hz-2098R ER-Alpha 雌激素受体α抗体
hz-0174R ER-alpha/beta 雌激素受体α/β抗体
hz-5896R WFDC5 P53应答基因蛋白5抗体
hz-5899R HIFPH4 缺氧诱导因子脯氨酰4羟化酶抗体
hz-5900R ADORA2B 腺苷A2b受体抗体（神经生长因子1受体）
hz-2927R OXSR1 氧化应激反应蛋白1抗体
hz-0116R ER-beta 雌激素受体β 抗体
hz-0255R ER β 雌激素受体β抗体
hz-5901R OS9 OS-9蛋白抗体
hz-5913R Calreticulin 钙网蛋白抗体
hz-5902R RBM3 RNA结合基因蛋白3抗体
hz-0593R EpCAM/CD326 上皮特异性抗原抗体
hz-3615R ESM1 内皮细胞特异性分子1抗体
hz-4101R ESPL1 外纺锤体极样蛋白1抗体
hz-5304R phospho-ESPL1 (Ser1073) 磷酸化外纺锤体极样蛋白1抗体
hz-5121R ERp29 内质网蛋白29抗体
hz-2928R PDLIM3/ALP 辅肌动蛋白相关LIM蛋白抗体
hz-5905R FLT3L FMS样酪氨酸激酶3配体抗体
hz-0188R ET-1 内皮素-1抗体
hz-0202R ET-1 内皮素-1抗体
hz-0954R ET-1 内皮素-1抗体
hzm-0954M ET-1(2C4) 内皮素-1单克隆抗体
hz-4198R ETBR/Endothelin B Receptor 内皮素B受体抗体
hz-3937R ETFB 电子转移黄素蛋白β肽/黄素蛋白抗体
关注
已认证
