蛋白激酶AKT底物1抗体

英文名称    PRAS40    

中文名称    蛋白激酶AKT底物1抗体    

别    名    AKT1 substrate 1 (proline rich); AKT1 substrate 1; AKT1-S1; AKT1S-1; AKT1S 1; AKT1S1; Lobe; PRAS 40; PRAS-40; PRAS40; Proline rich akt substrate; 40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 S1; AKT1 substrate 1; AKTS1_HUMAN; MGC2865; PRAS40; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1.    

蛋白激酶AKT底物1抗体      

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  免疫学  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    28kDa    

细胞定位    细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human PRAS40    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

蛋白激酶AKT底物1抗体产品介绍    background:
Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Function:
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Subunit:
Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins.

Subcellular Location:
Cytoplasm, cytosol.

Tissue Specificity:
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Post-translational modifications:
Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

Gene ID:
84335

Database links:

Entrez Gene: 84335 Human

Entrez Gene: 67605 Mouse

Entrez Gene: 292887 Rat

Omim: 610221 Human

SwissProt: Q96B36 Human

SwissProt: Q9D1F4 Mouse

Unigene: 515542 Human

Unigene: 148007 Mouse

Unigene: 102669 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

蛋白激酶AKT底物1抗体

hz-0360R-RBIThz Rabbit Anti-human sIgA/RBITC  罗丹明标记的兔抗人分泌型IgA

hz-0364R-RBIThz Rabbit Anti-human IgG F(ab')2/RBITC  罗丹明标记的兔抗人IgG F(ab')2 

hz-0364R-FIThz Rabbit Anti-human IgG F(ab')2/FITC  FITC标记的兔抗人IgG F(ab')2 

hz-0364R-Gold Rabbit Anti-human IgG F(ab')2/Gold  胶体金标记的兔抗人IgG F(ab')2 

hz-0364R-AP Rabbit Anti-human IgG F(ab')2/AP  碱性磷酸酶（AP）标记的兔抗人IgG F(ab')2 

hz-0364R-hzy3 Rabbit Anti-human IgG F(ab')2/Cy3  Cy3标记的兔抗人IgG F(ab')2 

hz-0364R-hzy5 Rabbit Anti-human IgG F(ab')2/Cy5  Cy5标记的兔抗人IgG F(ab')2 

hz-0364R-hzy5.5 Rabbit Anti-human IgG F(ab')2/Cy5.5  Cy5.5标记的兔抗人IgG F(ab')2 

hz-0364R-hzy7 Rabbit Anti-human IgG F(ab')2/Cy7  Cy7标记的兔抗人IgG F(ab')2 

hz-0364R-PE Rabbit Anti-human IgG F(ab')2/PE  PE标记的兔抗人IgG F(ab')2 

hz-0364R-PE-hzy3 Rabbit Anti-human IgG F(ab')2/PE-Cy3  PE-Cy3标记的兔抗人IgG F(ab')2 

hz-0364R-PE-hzY5 Rabbit Anti-human IgG F(ab')2/PE-CY5  PE-CY5标记的兔抗人IgG F(ab')2 

hz-0364R-APhz Rabbit Anti-human IgG F(ab')2/APC  APC标记的兔抗人IgG F(ab')2 

hz-0364R-AF350 Rabbit Anti-human IgG F(ab')2/Alexa Fluor 350  Alexa Fluor 350标记的兔抗人IgG F(ab')2 

hz-0364R-AF488 Rabbit Anti-human IgG F(ab')2/Alexa Fluor 488  Alexa Fluor 488标记的兔抗人IgG F(ab')2 

hz-0364R-AF555 Rabbit Anti-human IgG F(ab')2/Alexa Fluor 555  Alexa Fluor 555标记的兔抗人IgG F(ab')2 

hz-0364R-AF647 Rabbit Anti-human IgG F(ab')2/Alexa Fluor 647  Alexa Fluor 647标记的兔抗人IgG F(ab')2 

hz-0364R-PE-hzy5.5 Rabbit Anti-human IgG F(ab')2/PE-Cy5.5  PE-Cy5.5标记的兔抗人IgG F(ab')2 

hz-0364R-PE-hzy7 Rabbit Anti-human IgG F(ab')2/PE-Cy7  PE-Cy7标记的兔抗人IgG F(ab')2 

hz-0364R-Bio Rabbit Anti-human IgG F(ab')2/Bio  生物素标记的兔抗人IgG F(ab')2 

hz-0364R-HRP Rabbit Anti-human IgG F(ab')2/HRP  辣根过氧化物酶标记的兔抗人IgG F(ab')2 

hz-0377R-HRP Rabbit Anti-mouse IgG-Fc/HRP  辣根过氧化物酶标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzy3 Rabbit Anti-mouse IgG-Fc/PE-Cy3  PE-Cy3标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzY5 Rabbit Anti-mouse IgG-Fc/PE-CY5  PE-CY5标记的兔抗小鼠IgG-Fc

hz-0377R-APhz Rabbit Anti-mouse IgG-Fc/APC  APC标记的兔抗小鼠IgG-Fc

hz-0377R-AF350 Rabbit Anti-mouse IgG-Fc/Alexa Fluor 350  Alexa Fluor 350标记的兔抗小鼠IgG-Fc

hz-0377R-AF488 Rabbit Anti-mouse IgG-Fc/Alexa Fluor 488  Alexa Fluor 488标记的兔抗小鼠IgG-Fc

hz-0377R-AF555 Rabbit Anti-mouse IgG-Fc/Alexa Fluor 555  Alexa Fluor 555标记的兔抗小鼠IgG-Fc

hz-0377R-AF647 Rabbit Anti-mouse IgG-Fc/Alexa Fluor 647  Alexa Fluor 647标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzy5.5 Rabbit Anti-mouse IgG-Fc/PE-Cy5.5  PE-Cy5.5标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzy7 Rabbit Anti-mouse IgG-Fc/PE-Cy7  PE-Cy7标记的兔抗小鼠IgG-Fc

hz-0377R-AP Rabbit Anti-mouse IgG-Fc/AP  碱性磷酸酶（AP）标记的兔抗小鼠IgG-Fc

hz-0377R-Gold Rabbit Anti-mouse IgG-Fc/Gold  胶体金标记的兔抗小鼠IgG-Fc

hz-0377R-hzy3 Rabbit Anti-mouse IgG-Fc/Cy3  Cy3标记的兔抗小鼠IgG-Fc

hz-0377R-hzy5 Rabbit Anti-mouse IgG-Fc/Cy5  Cy5标记的兔抗小鼠IgG-Fc

hz-0377R-hzy5.5 Rabbit Anti-mouse IgG-Fc/Cy5.5  Cy5.5标记的兔抗小鼠IgG-Fc

hz-0377R-hzy7 Rabbit Anti-mouse IgG-Fc/Cy7  Cy7标记的兔抗小鼠IgG-Fc

hz-0377R-PE Rabbit Anti-mouse IgG-Fc/PE  PE标记的兔抗小鼠IgG-Fc

hz-0377R-FIThz Rabbit Anti-mouse IgG-Fc/FITC  FITC标记的兔抗小鼠IgG-Fc