丝氨酸/苏氨酸激酶p90RSK蛋白抗体

英文名称    RPS6KA1    

中文名称    丝氨酸/苏氨酸激酶p90RSK蛋白抗体    

别    名    RSK1 p90; 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein p90RSK; RSK1 p90; S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; KS6A1_HUMAN; MAP kinase activated protein kinase 1a; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1A; MAPKAPK-1a; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113; p90 RSK1; p90-RSK 1; p90rsk; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK-1; RSK1; S6K alpha 1; S6K-alpha-1.    

丝氨酸/苏氨酸激酶p90RSK蛋白抗体         

说 明 书    0.1ml  0.2ml      

研究领域    

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    81kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RPS6KA1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

丝氨酸/苏氨酸激酶p90RSK蛋白抗体    产品介绍    background:
Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.

Function:
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression.

Subunit:
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.

Subcellular Location:
Nucleus. Cytoplasm.

Post-translational modifications:
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.

Gene ID:
6195

Database links:

Entrez Gene: 6195 Human

Entrez Gene: 20111 Mouse

Entrez Gene: 81771 Rat

Omim: 601684 Human

SwissProt: Q15418 Human

SwissProt: P18653 Mouse

SwissProt: Q63531 Rat

Unigene: 149957 Human

Unigene: 301827 Mouse

Unigene: 34915 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.  丝氨酸/苏氨酸激酶p90RSK蛋白抗体      

hz-0294R-FIThz Rabbit Anti-Goat IgG/FITC  FITC标记的兔抗羊IgG

hz-0294R-hzy3 Rabbit Anti-Goat IgG/Cy3  Cy3标记的兔抗羊IgG

hz-0294R-hzy5 Rabbit Anti-Goat IgG/Cy5  Cy5标记的兔抗羊IgG

hz-0294R-hzy5.5 Rabbit Anti-Goat IgG/Cy5.5  Cy5.5标记的兔抗羊IgG

hz-0294R-hzy7 Rabbit Anti-Goat IgG/Cy7  Cy7标记的兔抗羊IgG

hz-0294R-PE Rabbit Anti-Goat IgG/PE  PE标记的兔抗羊IgG

hz-0294R-PE-hzy3 Rabbit Anti-Goat IgG/PE-Cy3  PE-Cy3标记的兔抗羊IgG

hz-0294R-AF350 Rabbit Anti-Goat IgG/Alexa Fluor 350  Alexa Fluor 350标记的兔抗羊IgG

hz-0294R-AF488 Rabbit Anti-Goat IgG/Alexa Fluor 488  Alexa Fluor 488标记的兔抗羊IgG

hz-0294R-AF555 Rabbit Anti-Goat IgG/Alexa Fluor 555  Alexa Fluor 555标记的兔抗羊IgG

hz-0294R-AF647 Rabbit Anti-Goat IgG/Alexa Fluor 647  Alexa Fluor 647标记的兔抗羊IgG

hz-0294R-PE-hzy5.5 Rabbit Anti-Goat IgG/PE-Cy5.5  PE-Cy5.5标记的兔抗羊IgG

hz-0294R-PE-hzy7 Rabbit Anti-Goat IgG/PE-Cy7  PE-Cy7标记的兔抗羊IgG

hz-0294R-Gold Rabbit Anti-Goat IgG/Gold  胶体金标记的兔抗羊IgG

hz-0294R-HRP Rabbit Anti-Goat IgG/HRP  辣根过氧化物酶标记的兔抗羊IgG

hz-0294R-RBIThz Rabbit Anti-Goat IgG/RBITC  罗丹明标记的兔抗羊IgG

hz-0294R-Bio Rabbit Anti-Goat IgG/Bio  生物素标记的兔抗羊IgG

hz-0370R-HRP Rabbit Anti-Goat IgM/HRP  辣根过氧化物酶标记的兔抗羊IgM

hz-0370R-Bio Rabbit Anti-Goat IgM/Bio  生物素标记的兔抗羊IgM

hz-0370R-AP Rabbit Anti-Goat IgM/AP  碱性磷酸酶（AP）标记的兔抗羊IgM

hz-0370R-Gold Rabbit Anti-Goat IgM/Gold  胶体金标记的兔抗羊IgM

hz-0370R-hzy3 Rabbit Anti-Goat IgM/Cy3  Cy3标记的兔抗羊IgM

hz-0370R-hzy5 Rabbit Anti-Goat IgM/Cy5  Cy5标记的兔抗羊IgM

hz-0370R-hzy5.5 Rabbit Anti-Goat IgM/Cy5.5  Cy5.5标记的兔抗羊IgM

hz-0370R-hzy7 Rabbit Anti-Goat IgM/Cy7  Cy7标记的兔抗羊IgM

hz-0370R-PE Rabbit Anti-Goat IgM/PE  PE标记的兔抗羊IgM

hz-0370R-PE-hzy3 Rabbit Anti-Goat IgM/PE-Cy3  PE-Cy3标记的兔抗羊IgM

hz-0370R-PE-hzY5 Rabbit Anti-Goat IgM/PE-CY5  PE-CY5标记的兔抗羊IgM

hz-0370R-APhz Rabbit Anti-Goat IgM/APC  APC标记的兔抗羊IgM

hz-0370R-AF350 Rabbit Anti-Goat IgM/Alexa Fluor 350  Alexa Fluor 350标记的兔抗羊IgM

hz-0370R-AF488 Rabbit Anti-Goat IgM/Alexa Fluor 488  Alexa Fluor 488标记的兔抗羊IgM

hz-0370R-AF555 Rabbit Anti-Goat IgM/Alexa Fluor 555  Alexa Fluor 555标记的兔抗羊IgM

hz-0370R-AF647 Rabbit Anti-Goat IgM/Alexa Fluor 647  Alexa Fluor 647标记的兔抗羊IgM

hz-0370R-PE-hzy5.5 Rabbit Anti-Goat IgM/PE-Cy5.5  PE-Cy5.5标记的兔抗羊IgM

hz-0370R-PE-hzy7 Rabbit Anti-Goat IgM/PE-Cy7  PE-Cy7标记的兔抗羊IgM

hz-0370R-RBIThz Rabbit Anti-Goat IgM/RBITC  罗丹明标记的兔抗羊IgM