PIAS-γ蛋白抗体

英文名称    PIASy    

中文名称    PIAS-γ蛋白抗体    

别    名    E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; PIAS4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6.    

PIAS-γ蛋白抗体        

说 明 书    0.1ml  0.2ml      

研究领域    细胞生物  细胞凋亡  生长因子和激素  转录调节因子  表观遗传学  泛素      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    55kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human PIASy    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PIAS-γ蛋白抗体 产品介绍    background:
The IL-6-type family of cytokines, which includes IL-6 and a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the STAT family are involved in IL-6 family-mediated signal transduction pathways, and upon activation undergo phosphorylation, dimerization and translocation to the nucleus. The duration and intensity of a cell's response to cytokines can be adjusted by the effect of several regulatory mechanisms. One example involves the protein inhibitor of activated signal transducer and activator of transcription (STAT) family (PIAS family) of proteins, which act as negative regulators of STATs in cytokine signaling. PIAS proteins are able to coactivate steroid receptor-dependent transcription as well. Human PIASy is a 510 amino acid transcriptional corepressor of the androgen receptor (AR). In addition, PIASy may regulate p53-mediated events and may direct p53 into a transactivation-independent mode of apoptosis.

Function:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.

Subcellular Location:
Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Tissue Specificity:
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Post-translational modifications:
Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.

Similarity:
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.

Gene ID:
51588

Database links:

Entrez Gene: 51588 Human

Entrez Gene: 59004 Mouse

Entrez Gene: 362827 Rat

Omim: 605989 Human

SwissProt: Q8N2W9 Human

SwissProt: Q3UGQ2 Mouse

SwissProt: Q9JM05 Mouse

Unigene: 105779 Human

Unigene: 34428 Mouse

Unigene: 12810 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

PIAS-γ蛋白抗体 

hz-0314R-hzy5 Rabbit Anti-chicken IgM/Cy5  Cy5标记的兔抗鸡IgM

hz-0314R-hzy5.5 Rabbit Anti-chicken IgM/Cy5.5  Cy5.5标记的兔抗鸡IgM

hz-0314R-hzy7 Rabbit Anti-chicken IgM/Cy7  Cy7标记的兔抗鸡IgM

hz-0314R-PE Rabbit Anti-chicken IgM/PE  PE标记的兔抗鸡IgM

hz-0314R-PE-hzy3 Rabbit Anti-chicken IgM/PE-Cy3  PE-Cy3标记的兔抗鸡IgM

hz-0314R-PE-hzY5 Rabbit Anti-chicken IgM/PE-CY5  PE-CY5标记的兔抗鸡IgM

hz-0314R-APhz Rabbit Anti-chicken IgM/APC  APC标记的兔抗鸡IgM

hz-0314R-HRP Rabbit Anti-chicken IgM/HRP  辣根过氧化物酶标记的兔抗鸡IgM

hz-0314R-AP Rabbit Anti-chicken IgM/AP  碱性磷酸酶（AP）标记的兔抗鸡IgM

hz-0314R-AF350 Rabbit Anti-chicken IgM/Alexa Fluor 350  Alexa Fluor 350标记的兔抗鸡IgM

hz-0314R-AF488 Rabbit Anti-chicken IgM/Alexa Fluor 488  Alexa Fluor 488标记的兔抗鸡IgM

hz-0314R-AF555 Rabbit Anti-chicken IgM/Alexa Fluor 555  Alexa Fluor 555标记的兔抗鸡IgM

hz-0314R-AF647 Rabbit Anti-chicken IgM/Alexa Fluor 647  Alexa Fluor 647标记的兔抗鸡IgM

hz-0314R-PE-hzy5.5 Rabbit Anti-chicken IgM/PE-Cy5.5  PE-Cy5.5标记的兔抗鸡IgM

hz-0314R-PE-hzy7 Rabbit Anti-chicken IgM/PE-Cy7  PE-Cy7标记的兔抗鸡IgM

hz-0314R-Bio Rabbit Anti-chicken IgM/Bio  生物素标记的兔抗鸡IgM

hz-0303R-Bio Rabbit Anti-dog IgG/Bio  生物素标记的兔抗狗IgG

hz-0303R-HRP Rabbit Anti-dog IgG/HRP  辣根过氧化物酶标记的兔抗狗IgG

hz-0303R-AP Rabbit Anti-dog IgG/AP  碱性磷酸酶（AP）标记的兔抗狗IgG

hz-0303R-hzy3 Rabbit Anti-dog IgG/Cy3  Cy3标记的兔抗狗IgG

hz-0303R-hzy5 Rabbit Anti-dog IgG/Cy5  Cy5标记的兔抗狗IgG

hz-0303R-hzy5.5 Rabbit Anti-dog IgG/Cy5.5  Cy5.5标记的兔抗狗IgG

hz-0303R-hzy7 Rabbit Anti-dog IgG/Cy7  Cy7标记的兔抗狗IgG

hz-0303R-PE Rabbit Anti-dog IgG/PE  PE标记的兔抗狗IgG

hz-0303R-PE-hzy3 Rabbit Anti-dog IgG/PE-Cy3  PE-Cy3标记的兔抗狗IgG

hz-0303R-PE-hzY5 Rabbit Anti-dog IgG/PE-CY5  PE-CY5标记的兔抗狗IgG

hz-0303R-APhz Rabbit Anti-dog IgG/APC  APC标记的兔抗狗IgG

hz-0303R-AF350 Rabbit Anti-dog IgG/Alexa Fluor 350  Alexa Fluor 350标记的兔抗狗IgG

hz-0303R-AF647 Rabbit Anti-dog IgG/Alexa Fluor 647  Alexa Fluor 647标记的兔抗狗IgG

hz-0303R-PE-hzy5.5 Rabbit Anti-dog IgG/PE-Cy5.5  PE-Cy5.5标记的兔抗狗IgG

hz-0303R-PE-hzy7 Rabbit Anti-dog IgG/PE-Cy7  PE-Cy7标记的兔抗狗IgG

hz-0303R-RBIThz Rabbit Anti-dog IgG/RBITC  罗丹明标记的兔抗狗IgG

hz-0303R-Gold Rabbit Anti-dog IgG/Gold  胶体金标记的兔抗狗IgG

hz-0303R-FIThz Rabbit Anti-dog IgG/FITC  FITC标记的兔抗狗IgG

hz-0303R-AF488 Rabbit Anti-dog IgG/Alexa Fluor 488  Alexa Fluor 488标记的兔抗狗IgG

hz-0303R-AF555 Rabbit Anti-dog IgG/Alexa Fluor 555  Alexa Fluor 555标记的兔抗狗IgG

hz-0294R-PE-hzY5 Rabbit Anti-Goat IgG/PE-CY5  PE-CY5标记的兔抗羊IgG

hz-0294R-APhz Rabbit Anti-Goat IgG/APC  APC标记的兔抗羊IgG

hz-0294R-AP Rabbit Anti-Goat IgG/AP  碱性磷酸酶（AP）标记的兔抗羊IgG