磷脂酰肌醇激酶（PI3Kβ）抗体

英文名称    PI3 Kinase p110 beta    

中文名称    磷脂酰肌醇激酶（PI3Kβ）抗体    

别    名    p110 BETA; p110Beta; PI3K beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic beta polypeptide; PI3 kinase p110 subunit beta; PI3-kinase subunit beta; PI3K; PI3K beta; PI3K-beta; PI3Kbeta; PI3KCB; PIK3C1; Pik3cb; PK3CB_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; 5-bisphosphate 3-kinase catalytic subunit beta isoform.    

磷脂酰肌醇激酶（PI3Kβ）抗体        

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  细胞生物  免疫学  信号转导  细胞周期蛋白  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    123kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human PI3 Kinase p110 beta    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷脂酰肌醇激酶（PI3Kβ）抗体  产品介绍    background:
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011].

Function:
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.

Subunit:
Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export. Part of a complex with PIK3R1 and PTEN. Binding to PTEN may antagonize the lipid kinase activity under normal growth conditions. Part of a complex involved in autophagosome formation composed of PIK3C3 and PIK3R4. Interacts with BECN1, ATG14 and RAB5A.

Subcellular Location:
Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export.

Tissue Specificity:
Expressed ubiquitously.

Post-translational modifications:
Phosphorylation at Ser-1070 down-regulates lipid kinase activity. 

Similarity:
Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.

Gene ID:
5291

Database links:

Entrez Gene: 5291 Human

Entrez Gene: 74769 Mouse

Entrez Gene: 85243 Rat

Omim: 602925 Human

SwissProt: P42338 Human

SwissProt: Q24JU2 Human

SwissProt: Q3U4Q1 Mouse

SwissProt: Q8BTI9 Mouse

SwissProt: Q9Z1L0 Rat

Unigene: 239818 Human

Unigene: 213128 Mouse

Unigene: 44268 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷脂酰肌醇激酶（PI3Kβ）抗体  

hz-0293M-FIThz Mouse Anti-rat IgG/FITC  FITC标记的小鼠抗大鼠IgG

hz-0293M-HRP Mouse Anti-rat IgG/HRP  辣根过氧化物酶标记的小鼠抗大鼠IgG

hz-0293M-RBIThz Mouse Anti-rat IgG/RBITC  罗丹明标记的小鼠抗大鼠IgG

hz-0293M-Bio Mouse Anti-rat IgG/Bio  生物素标记的小鼠抗大鼠IgG

hz-0293M-PE-hzy7 Mouse Anti-rat IgG/PE-Cy7  PE-Cy7标记的小鼠抗大鼠IgG

hz-0326M-Bio Mouse Anti-Bov IgG/Bio  生物素标记的小鼠抗牛IgG

hz-0326M-hzy3 Mouse Anti-Bov IgG/Cy3  Cy3标记的小鼠抗牛IgG

hz-0326M-AP Mouse Anti-Bov IgG/AP  碱性磷酸酶（AP）标记的小鼠抗牛IgG

hz-0326M-PE-hzy7 Mouse Anti-Bov IgG/PE-Cy7  PE-Cy7标记的小鼠抗牛IgG

hz-0326M-Gold Mouse Anti-Bov IgG/Gold  胶体金标记的小鼠抗牛IgG

hz-0326M-HRP Mouse Anti-Bov IgG/HRP  辣根过氧化物酶标记的小鼠抗牛IgG

hz-0326M-RBIThz Mouse Anti-Bov IgG/RBITC  罗丹明标记的小鼠抗牛IgG

hz-0326M-FIThz Mouse Anti-Bov IgG/FITC  FITC标记的小鼠抗牛IgG

hz-0326M-hzy5 Mouse Anti-Bov IgG/Cy5  Cy5标记的小鼠抗牛IgG

hz-0326M-hzy5.5 Mouse Anti-Bov IgG/Cy5.5  Cy5.5标记的小鼠抗牛IgG

hz-0326M-hzy7 Mouse Anti-Bov IgG/Cy7  Cy7标记的小鼠抗牛IgG

hz-0326M-PE Mouse Anti-Bov IgG/PE  PE标记的小鼠抗牛IgG

hz-0326M-PE-hzy3 Mouse Anti-Bov IgG/PE-Cy3  PE-Cy3标记的小鼠抗牛IgG

hz-0326M-PE-hzY5 Mouse Anti-Bov IgG/PE-CY5  PE-CY5标记的小鼠抗牛IgG

hz-0326M-APhz Mouse Anti-Bov IgG/APC  APC标记的小鼠抗牛IgG

hz-0326M-AF350 Mouse Anti-Bov IgG/Alexa Fluor 350  Alexa Fluor 350标记的小鼠抗牛IgG

hz-0326M-AF488 Mouse Anti-Bov IgG/Alexa Fluor 488  Alexa Fluor 488标记的小鼠抗牛IgG

hz-0326M-AF555 Mouse Anti-Bov IgG/Alexa Fluor 555  Alexa Fluor 555标记的小鼠抗牛IgG

hz-0326M-AF647 Mouse Anti-Bov IgG/Alexa Fluor 647  Alexa Fluor 647标记的小鼠抗牛IgG

hz-0326M-PE-hzy5.5 Mouse Anti-Bov IgG/PE-Cy5.5  PE-Cy5.5标记的小鼠抗牛IgG

hz-0358M-hzy3 Mouse Anti-Guinea pig IgG/Cy3  Cy3标记的小鼠抗豚鼠IgG

hz-0358M-hzy5 Mouse Anti-Guinea pig IgG/Cy5  Cy5标记的小鼠抗豚鼠IgG

hz-0358M-hzy5.5 Mouse Anti-Guinea pig IgG/Cy5.5  Cy5.5标记的小鼠抗豚鼠IgG

hz-0358M-hzy7 Mouse Anti-Guinea pig IgG/Cy7  Cy7标记的小鼠抗豚鼠IgG

hz-0358M-PE Mouse Anti-Guinea pig IgG/PE  PE标记的小鼠抗豚鼠IgG

hz-0358M-PE-hzy3 Mouse Anti-Guinea pig IgG/PE-Cy3  PE-Cy3标记的小鼠抗豚鼠IgG

hz-0358M-PE-hzY5 Mouse Anti-Guinea pig IgG/PE-CY5  PE-CY5标记的小鼠抗豚鼠IgG

hz-0358M-FIThz Mouse Anti-Guinea pig IgG/FITC  FITC标记的小鼠抗豚鼠IgG

hz-0358M-RBIThz Mouse Anti-Guinea pig IgG/RBITC  罗丹明标记的小鼠抗豚鼠IgG

hz-0358M-AP Mouse Anti-Guinea pig IgG/AP  碱性磷酸酶（AP）标记的小鼠抗豚鼠IgG

hz-0358M-Bio Mouse Anti-Guinea pig IgG/Bio  生物素标记的小鼠抗豚鼠IgG

hz-0358M-Gold Mouse Anti-Guinea pig IgG/Gold  胶体金标记的小鼠抗豚鼠IgG

hz-0358M-HRP Mouse Anti-Guinea pig IgG/HRP  辣根过氧化物酶标记的小鼠抗豚鼠IgG