肿瘤错配修复基因PMS2抗体

英文名称    PMS2    

中文名称    肿瘤错配修复基因PMS2抗体    

别    名    DNA mismatch repair protein PMS2; Mismatch repair endonuclease PMS2; Mismatch repair gene PMSL2; PMS2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS2_HUMAN; Postmeiotic segregation increased, S. cerevisiae, 2; DNA mismatch repair gene homologue; H_DJ0042M02.9; HNPCC4; PMS 2; PMS1 protein homolog 2; PMS2 postmeiotic segregation increased 2; PMS2CL; PMSL2.    

肿瘤错配修复基因PMS2抗体        

说 明 书    0.1ml  0.2ml      

研究领域    

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    95kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human PMS2    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

肿瘤错配修复基因PMS2抗体 产品介绍    background:
This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2008].

Function:
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Subunit:
Heterodimer of PMS2 and MLH1 (MutL alpha). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1.

Subcellular Location:
Nucleus.

DISEASE:
Hereditary non-polyposis colorectal cancer 4 (HNPCC4) [MIM:614337]: An autosomal dominant disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=The disease is caused by mutations affecting the gene represented in this entry. 
Mismatch repair cancer syndrome (MMRCS) [MIM:276300]: Autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the DNA mismatch repair MutL/HexB family.

Gene ID:
5395

Database links:

Entrez Gene: 441194 Human

Entrez Gene: 5395 Human

Entrez Gene: 18861 Mouse

Omim: 600259 Human

SwissProt: P54278 Human

SwissProt: P54279 Mouse

Unigene: 632637 Human

Unigene: 2950 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

肿瘤错配修复基因PMS2抗体 

hz-0358G-AF647 Goat Anti-Guinea pig IgG/Alexa Fluor 647  Alexa Fluor 647标记的羊抗豚鼠IgG

hz-0358G-PE-hzy5.5 Goat Anti-Guinea pig IgG/PE-Cy5.5  PE-Cy5.5标记的羊抗豚鼠IgG

hz-0358G-PE-hzy7 Goat Anti-Guinea pig IgG/PE-Cy7  PE-Cy7标记的羊抗豚鼠IgG

hz-0295G-HRP Goat Anti-rabbit IgG/HRP  辣根过氧化物酶标记的羊抗兔IgG

hz-0295G-hzy7 Goat Anti-rabbit IgG/Cy7  Cy7标记的羊抗兔IgG

hz-0295G-hzy5 Goat Anti-rabbit IgG/Cy5  Cy5标记的羊抗兔IgG

hz-0295G-PE-hzY5 Goat Anti-rabbit IgG/PE-CY5  PE-CY5标记的羊抗兔IgG

hz-0295G-PE Goat Anti-rabbit IgG/PE  PE标记的羊抗兔IgG

hz-0295G-hzy3 Goat Anti-rabbit IgG/Cy3  Cy3标记的羊抗兔IgG

hz-0295G-hzy5.5 Goat Anti-rabbit IgG/Cy5.5  Cy5.5标记的羊抗兔IgG

hz-0295G-PE-hzy3 Goat Anti-rabbit IgG/PE-Cy3  PE-Cy3标记的羊抗兔IgG

hz-0295G-APhz Goat Anti-rabbit IgG/APC  APC标记的羊抗兔IgG

hz-0295G-AF350 Goat Anti-rabbit IgG/Alexa Fluor 350  Alexa Fluor 350标记的羊抗兔IgG

hz-0295G-AF488 Goat Anti-rabbit IgG/Alexa Fluor 488  Alexa Fluor 488标记的羊抗兔IgG

hz-0295G-PE-hzy5.5 Goat Anti-rabbit IgG/PE-Cy5.5  PE-Cy5.5标记的羊抗兔IgG

hz-0295G-AF555 Goat Anti-rabbit IgG/Alexa Fluor 555  Alexa Fluor 555标记的羊抗兔IgG

hz-0295G-AF647 Goat Anti-rabbit IgG/Alexa Fluor 647  Alexa Fluor 647标记的羊抗兔IgG

hz-0295G-PE-hzy7 Goat Anti-rabbit IgG/PE-Cy7  PE-Cy7标记的羊抗兔IgG

hz-0295G-Bio Goat Anti-rabbit IgG/Bio  生物素标记的羊抗兔IgG

hz-0295G-AP Goat Anti-rabbit IgG/AP  碱性磷酸酶（AP）标记的羊抗兔IgG

hz-0295G-Gold Goat Anti-rabbit IgG/Gold  胶体金标记的羊抗兔IgG

hz-0295G-FIThz Goat Anti-rabbit IgG/FITC  FITC标记的羊抗兔IgG

hz-0295G-RBIThz Goat Anti-rabbit IgG/RBITC  罗丹明标记的羊抗兔IgG

hz-0310G-RBIThz Goat Anti-Chicken IgG/RBITC  罗丹明标记的羊抗鸡IgG

hz-0310G-Gold Goat Anti-Chicken IgG/Gold  胶体金标记的羊抗鸡IgG

hz-0310G-FIThz Goat Anti-Chicken IgG/FITC  FITC标记的羊抗鸡IgG

hz-0310G-hzy3 Goat Anti-Chicken IgG/Cy3  Cy3标记的羊抗鸡IgG

hz-0310G-hzy5 Goat Anti-Chicken IgG/Cy5  Cy5标记的羊抗鸡IgG

hz-0310G-hzy5.5 Goat Anti-Chicken IgG/Cy5.5  Cy5.5标记的羊抗鸡IgG

hz-0310G-hzy7 Goat Anti-Chicken IgG/Cy7  Cy7标记的羊抗鸡IgG

hz-0310G-PE Goat Anti-Chicken IgG/PE  PE标记的羊抗鸡IgG

hz-0310G-PE-hzy3 Goat Anti-Chicken IgG/PE-Cy3  PE-Cy3标记的羊抗鸡IgG

hz-0310G-PE-hzY5 Goat Anti-Chicken IgG/PE-CY5  PE-CY5标记的羊抗鸡IgG

hz-0310G-APhz Goat Anti-Chicken IgG/APC  APC标记的羊抗鸡IgG

hz-0310G-AF350 Goat Anti-Chicken IgG/Alexa Fluor 350  Alexa Fluor 350标记的羊抗鸡IgG