磷酸化前病毒整合位点蛋白1抗体

英文名称    Phospho-PIM1(Ser99)    

中文名称    磷酸化前病毒整合位点蛋白1抗体    

别    名    PIM1 (phospho S99); PIM1 (phospho Ser99); Oncogene PIM 1; Oncogene PIM1; PIM 1; Pim 1 kinase; Pim 1 oncogene; PIM; Pim2; PIM3; Proto oncogene serine/threonine protein kinase Pim 1; Proviral integration site 1; Proviral integration site 2.    

磷酸化前病毒整合位点蛋白1抗体 

说 明 书    0.1ml      

研究领域    肿瘤  免疫学  信号转导  干细胞  细胞凋亡  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Guinea Pig,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    44kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Phospho-PIM1(Ser99)    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化前病毒整合位点蛋白1抗体产品介绍    background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]

Function:
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

Subunit:
Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with CDKN1B and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Interacts (ubiquitinated form) with HSP70 and promotes its proteosomal degradation. Interacts with CDKN1A. Interacts with CDC25C. Interacts (via N-terminal 96 residues) with CDC25A. Interacts with MAP3K5. Interacts with MYC.

Subcellular Location:
Isoform 2: Cytoplasm. Nucleus. Isoform 1: Cell membrane.

Tissue Specificity:
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

Post-translational modifications:
Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation. 
Ubiquitinated, leading to proteasomal degradation.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily. 
Contains 1 protein kinase domain.

Gene ID:
5292

Database links:

Entrez Gene: 5292 Human

Entrez Gene: 18712 Mouse

Entrez Gene: 24649 Rat

Omim: 164960 Human

SwissProt: P11309 Human

SwissProt: P06803 Mouse

SwissProt: P26794 Rat

Unigene: 81170 Human

Unigene: 405293 Mouse

Unigene: 485038 Mouse

Unigene: 34888 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化前病毒整合位点蛋白1抗体

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hz-5705R LZTS1  假定肿瘤抑制亮氨酸拉链蛋白1抗体

hz-5759R PPP2R3  蛋白质磷酸酶2A亚基3抗体

hz-2922R AHSG/Fetuin A/Alpha 2 HS Glycoprotein  胎球蛋白A

hz-5793R Protein C inhibitor/SerpinA5  蛋白C抑制因子抗体

hz-5794R Robo  轴突导向受体抗体

hz-5795R Robo4  肿瘤血管内皮调节蛋白Robo4抗体

hz-2925R Klotho  Klotho多肽蛋白抗体

hz-5837R EPDR1  哺乳动物室管膜相关蛋白1抗体

hz-5862R ADAMTSL2  整合素样金属蛋白酶与凝血酶样2蛋白抗体

hz-5885R Myosin heavy chain 1  肌球蛋白重链抗体

hz-5893R TBCD4/TBC1D4  TBC结构域TBCD4蛋白抗体

hz-5906R FGF9  碱性成纤维细胞生长因子9抗体

hz-5943R SET translocation  SET易位蛋白抗体（髓系白血病相关蛋白）

hz-2189R MCKD2/UMOD  尿调节蛋白抗体

hz-0916R MCKD2/UMOD  尿调节蛋白抗体

hzm-0910M Batroxobin(5H4)  巴曲霉毒素单克隆抗体

hz-0910R Batroxobin Protein  蛇毒巴曲酶抗体

hz-0911R Batroxobin  蛇毒巴曲酶抗体

hz-4603R TRIP  肿瘤坏死因子受体相互作用蛋白抗体

hz-2915R HOXD10  同源盒蛋白HOXD10抗体

hz-0291R TG  兔抗甲状腺球蛋白

hz-5401R Phospho-c-Kit(Ser721)  磷酸化原癌基因c-kit抗体

hz-6445R PRLR/Prolactin Receptor  泌乳素受体抗体

hz-7949R DAO1/ABP1  阿米洛利结合蛋白1抗体

二、标记一抗

编  号 名  称

三、二抗

编  号 名  称

hz-0373R Dog IgM  兔抗犬IgM抗体

hz-0326Gs Goat anti-Bovine IgG whole serum  羊抗牛IgG抗血清

hz-0296Gs Goat anti-Mouse IgG whole serum  羊抗小鼠IgG抗血清

hz-0368Gs Goat anti-Mouse IgM whole serum  羊抗小鼠IgM抗血清

hz-0293Gs Goat anti-rat IgG whole serum  羊抗大鼠IgG抗血清

hz-0358Gs Goat anti-Gpig IgG whole serum  羊抗豚鼠IgG抗血清

hz-0295Gs Goat anti-Rabbit IgG whole serum  羊抗兔IgG抗血清

hz-0297Gs Goat anti-human IgG whole serum  羊抗人IgG抗血清