Rho相关蛋白激酶1抗体

英文名称    ROCK1    

中文名称    Rho相关蛋白激酶1抗体    

别    名    p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.    

Rho相关蛋白激酶1抗体        

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  细胞生物  信号转导  细胞周期蛋白  激酶和磷酸酶  细胞骨架      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Rabbit,     

产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    158kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ROCK1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

Rho相关蛋白激酶1抗体产品介绍    background:
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].

Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation.

Subunit:
Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.

Tissue Specificity:
Detected in blood platelets.

Post-translational modifications:
Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.

Gene ID:
6093

Database links:

Entrez Gene: 6093 Human

Entrez Gene: 19877 Mouse

Entrez Gene: 81762 Rat

Omim: 601702 Human

SwissProt: Q8MIT6 Cow

SwissProt: Q13464 Human

SwissProt: P70335 Mouse

SwissProt: Q63644 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.     

Rho相关蛋白激酶1抗体

hz-5005R PFKFB2  果糖-2,6-二磷酸酶心肌酶抗体

hz-0796R Fascin1  纤维束1抗体

hz-0772R phospho-FSCN1(Ser39)  磷酸化纤维束蛋白同源物1抗体

hz-4159R PIK3C3/PI3 kinase type 3  磷脂酰肌醇激酶3催化亚单位3抗体

hz-5579R phospho-PIK3C3(Ser282)  磷酸化磷脂酰肌醇激酶3催化亚单位3抗体

hz-5006R PGAM1/PGAM2  磷酸变位酶1抗体

hz-2335R PGK1  磷酸激酶1抗体

hz-5580R phospho-PIK3C3(Ser676)  磷酸化磷脂酰肌醇激酶3催化亚单位3抗体

hz-1551R PG-C/Pepsinogen 2  胃蛋白酶原C抗体

hz-5007R PGLS  磷酸葡萄糖酸内酯酶抗体

hz-5581R phospho-PIK3C3(Ser164)  磷酸化磷脂酰肌醇激酶3催化亚单位3抗体

hz-5009R PHKG1  磷酸化酶激酶γ1

hz-5010R PHKG2  磷酸化酶激酶γ2

hz-5008R PHKA1  磷酸激酶α1抗体

hz-4032R PHKB  磷酸化酶β抗体

hz-1832R PPARGC1A/PGC1 α  过氧化物酶体增殖物激活受体γ辅激活子1α抗体

hz-5091R PHYH  植烷酸氧化酶抗体

hz-0823R PGRN/Granulin  颗粒蛋白前体抗体

hz-3806R UCHL1/PGP9.5  神经细胞胞浆蛋白9.5抗体（蛋白基因产物9.5）

hz-1449R Prohibitin  抑制素/肿瘤抑制因子抗体

hz-0128R PI3K/PI3 kinase p85 alpha subunit  磷脂酰肌醇激酶抗体

hz-3332R phospho-PI3K p85(Tyr458)/p55(Tyr199)  磷酸化磷脂酰肌醇激酶抗体

hz-5538R phospho-PI3K p85/PIK3R1(Tyr368)  磷酸化磷脂酰肌醇激酶抗体

hz-5571R phospho-PIK3R1(Tyr467)  磷酸化磷脂酰肌醇激酶抗体

hz-2067R PIK3CA  磷脂酰肌醇激酶催化亚单位A抗体

hz-3593R PI3 Kinase p110 delta  磷脂酰肌醇激酶催化亚单位D抗体

hz-5572R phospho-PIK3R1(Tyr556)  磷酸化磷脂酰肌醇激酶抗体

hz-5570R phospho-PI3KCA(Tyr317)  磷酸化磷脂酰肌醇激酶催化亚单位A抗体

hz-5587R phospho-PI3K/PIK3CD(Tyr524)  磷酸化磷脂酰肌醇激酶催化亚单位D抗体

hz-0420R PIBF  孕激素诱导阻断因子抗体

hz-4670R PILR beta  细胞表面受体PILRβ抗体

hz-2690R PILR beta  细胞表面受体PILRβ抗体

hz-3540R PIM1  丝氨酸/苏氨酸激酶蛋白Pim1抗体

hz-3353R Phospho-PIM1(Tyr218)  磷酸化前病毒整合位点蛋白1抗体

hz-1872R PIRH2  泛素连接酶抗体

hz-3541R Pin1  肽基脯氨酞顺反异构酶Pinl抗体