维甲酸受体RAR α/RAR α抗体

英文名称    RAR alpha    

中文名称    维甲酸受体RAR α/RAR α抗体    

别    名    Acute Promelocytic Leukemia Breakpoint Cluster Region; Acute Promelocytic Leukemia Breakpoint Cluster Region; NR1B1; Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein; Nuclear receptor subfamily 1 group B member 1; Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form; RAR A; RAR alpha; RAR alpha; RAR alpha form; RAR alpha form; RAR; RAR-alpha; RARA; RARA; RARA protein; RARA/PML Fusion Gene; RARA/PML Fusion Gene; RARA_HUMAN; RARalpha; RARalpha1; Retinoic acid receptor alpha; Retinoic acid receptor alpha polypeptide    

维甲酸受体RAR α/RAR α抗体       

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    48kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Retinoic acid Receptor Alpha    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

维甲酸受体RAR α/RAR α抗体产品介绍    background:
Retinoic acid receptor alpha (RAR-alpha) is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression. Subunit: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes.

Function:
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.

Subunit:
Heterodimer; with RXRA. Binds DNA preferentially as a heterodimer. Interacts with CDK7 By similarity. Interacts with coactivators NCOA3 and NCOA6. Interacts with NCOA7; the interaction requires ligand-binding. Interacts with MLL5. Interacts (via the ligand-binding domain) with PRAME; the interaction is ligand (retinoic acid)-dependent. Interacts with AKT1; the interaction phosphorylates RARA and represses transactivation. Interacts with PRKAR1A; the interaction negatively regulates RARA transcriptional activity. Interacts with NCOR1 and NCOR2. Interacts with PRMT2. Interacts with LRIF1. Interacts with ASXL1 and NCOA1.

Subcellular Location:
Nucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.

Post-translational modifications:
Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity. Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. 
Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. 
Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. 
Ubiquitinated.

DISEASE:
Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

Gene ID:
5914

Database links:
Entrez Gene: 5914 Human 
Entrez Gene: 19401 Mouse
Entrez Gene: 24705 Rat
Omim: 180240 Human
SwissProt: P10276 Human
SwissProt: P11416 Mouse
Unigene: 654583 Human
Unigene: 439744 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.     

维甲酸受体RAR α/RAR α抗体

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hz-5679R phospho-RPS6KA1(Thr359)  磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

hz-5680R phospho-RPS6KA1(Thr348)  磷酸化磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

hz-5681R phospho-RPS6KA1(Ser363)  磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

hz-5682R phospho-RPS6KA1(Ser352)  磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

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hz-0198R PACAP R1  腺苷酸环化酶激活肽受体1抗体

hz-0190R PACAP-27/38  腺苷酸环化酶激活肽-27/38抗体

hz-0200R PACAP-38  腺苷酸环化酶激活肽-38抗体

hz-3833R PABP  多聚腺苷酸结合蛋白抗体

hz-3836R PABP (Methyl Arg455/460)  甲基化多聚腺苷酸结合蛋白抗体

hz-0318R PADI4  关节炎相关基因抗体

hz-1119R PAF  血小板活化因子抗体

hz-0588R PALB2  乳腺癌易感基因相关蛋白2

hz-1451R PAFAH2/Lp-PLA2  脂蛋白磷脂酶A2抗体

hz-1478R PAFR/PTAFR  血小板活化因子受体抗体

hz-2277R PAK1  p21激活激酶1抗体

hz-3518R PAK1  p21激活激酶1抗体

hz-3520R PAK2  p21激活激酶2抗体

hz-1704R PAI-1  纤溶酶原激活物抑制因子抗体

hz-3517R PAK4  p21激活激酶4抗体

hz-3314R Phospho-PAK4(Ser474)/PAK5(Ser602)/PAK6(Ser560)  磷酸化p21激活激酶4/5/6抗体

hz-2270R Phospho-PAK4(Ser99)  磷酸化p21激活激酶4抗体

hz-3517M PAK4  小鼠抗p21激活激酶4抗体

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hz-3315R Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197)  磷酸化p21激活激酶1/2抗体

hz-3316R Phospho-PAK1(Thr423)/PAK2 (Thr402)  磷酸化p21激活激酶1/2抗体

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hz-1652R phospho-PAK1/2/3(Thr423)  磷酸化p21激活激酶1/2/3抗体

hz-0655R PAK7/PAK5/MBT  激活激酶PAK7抗体

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