细胞周期检控Rad17蛋白抗体（复制因子C）

英文名称    Rad17    

中文名称    细胞周期检控Rad17蛋白抗体（复制因子C）    

别    名    RFC; CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 (S. pombe) homolog; RAD17; RAD17 homolog; Rad17 like protein; RAD17_HUMAN; RAD17Sp; Rad24; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog.    

细胞周期检控Rad17蛋白抗体（复制因子C）            

说 明 书    0.1ml  0.2ml      

研究领域    免疫学  染色质和核信号  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    77kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Rad17    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

细胞周期检控Rad17蛋白抗体（复制因子C）    产品介绍    background:
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013].

Function:
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and C12orf32/RHINO onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. 

Subunit:
Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Interacts with RAD1 and RAD9 within the RAD1-RAD9-HUS1 complex. Interacts with RAD9B, POLE, NHP2L1 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the RAD1-RAD9-HUS1 complex.

Subcellular Location:
Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

Tissue Specificity:
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).

Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. 

Similarity:
Belongs to the rad17/RAD24 family. 

Gene ID:
5884

Database links:

Entrez Gene: 5884 Human

Entrez Gene: 19356 Mouse

Omim: 603139 Human

SwissProt: O75943 Human

SwissProt: Q6NXW6 Mouse

Unigene: 16184 Human

Unigene: 248489 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

细胞周期检控Rad17蛋白抗体（复制因子C）    

hz-2314R APOA1  载脂蛋白A1抗体

hz-1498R FASN/Fatty Acid Synthase 1  脂肪酸合成酶抗体

hz-5034R APOA2  载脂蛋白A2抗体

hz-2688R ApoA4  载脂蛋白A4抗体

hz-1765R FAST kinase  Fas活化的丝/苏氨酸激酶抗体

hz-5036R APOC2  载脂蛋白C2抗体

hz-5037R APOC3  载脂蛋白C3抗体

hz-3876R APOA4  载脂蛋白A4抗体

hz-5035R APOA5  载脂蛋白A5抗体

hz-5928R BASC4  乳腺癌扩增序列蛋白4抗体

hz-5959R CASC5  肿瘤易感性候选基因5抗体

hz-0216R FasL/CD178  兔抗FasL抗体。

hz-2260R fatty acid elongase  脂肪酸延长酶抗体

hz-2573R Fc γ RII a/CD32  免疫球蛋白G Fc段受体Ⅱ抗体

hz-4991R Fc γ RII a/CD32  免疫球蛋白G Fc段受体Ⅱ抗体

hz-6031R phospho-CD32B(Tyr291)  磷酸化CD32B抗体

hz-5930R Centaurin γ1  中心体肌动蛋白γ1抗体（增强型PI3K蛋白）

hz-1157R FBN1  原纤维蛋白1抗体

hz-3980R FBP1  果糖1，6-二磷酸酯酶抗体

hz-3981R FBP2  肌肉果糖二磷酸酶抗体

hz-1766R FDC-SP/ADC  滤泡树突细胞分泌蛋白抗体

hz-5931R DCUN1D2  DCN1样蛋白2抗体

hz-0110R APBB1/Fe65 protein  铁蛋白Fe65抗体

hz-5165R phospho-APBB1 (Ser175)  磷酸化铁蛋白Fe65抗体

hz-5166R phospho-APBB1 (Ser347)  磷酸化铁蛋白Fe65抗体

hz-3938R Fumarate hydratase/FH  富马酸水合酶抗体

hz-0229R FGF1/AFGF  酸性成纤维细胞生长因子抗体

hz-5932R GCET1  B淋巴细胞生发中心相关蛋白抗体

hz-1255R FGF3/Oncogene INT2  纤维母细胞生长因子3抗体

hz-1256R FGF4  纤维母细胞生长因子4抗体

hz-1257R FGF5  纤维母细胞生长因子5抗体

hz-5923R FGF6  纤维母细胞生长因子6抗体

hz-5933R KCNK  TWIK相关酸敏感钾离子通道蛋白9抗体

hz-5934R KDM4C/GASC1/JMJD2C  鳞状细胞癌增强蛋白1抗体

hz-0734R KGF/FGF7  纤维母细胞生长因子7抗体

hz-0735R FGF8/HBGF-8  成纤维细胞生长因子8抗体

hz-5935R KLHL7  kelch样蛋白7抗体