磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体

英文名称    phospho-RPS6KA1(Thr359)    

中文名称    磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体    

别    名    RPS6KA1(phospho T359); p90 RSK1 ; p90rsk ; p90S6K ; pp90RSK1 ; Ribosomal protein S6 kinase 90kD 1 ; Ribosomal protein S6 kinase 90kD polypeptide 1 ; Ribosomal protein S6 kinase 90kDa polypeptide 1 ; Ribosomal protein S6 kinase alpha 1 ; Ribosomal protein S6 kinase polypeptide 1 ; Ribosomal S6 kinase 1 ; RPS6K1 alpha ; rps6ka ; Rps6ka1 ; RSK 1 ; RSK 1 p90 ; RSK ; RSK1 ; S6K alpha 1 ; 90 kDa ribosomal protein S6 kinase 1 ; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 ; dJ590P13.1 ; EC 2.7.11.1 ; HU 1 ; HU1 ; MAP kinase activated protein kinase 1a ; MAPKAP kinase 1A ; MAPKAPK1A ; MGC79981 ; Mitogen-activated protein kinase-activated protein kinase 1A; KS6A1_HUMAN.    

磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体         

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  信号转导  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    81kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Thr359    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体 产品介绍    background:
Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.

Function:
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression.

Subunit:
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.

Subcellular Location:
Nucleus. Cytoplasm.

Post-translational modifications:
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1. 
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. 
Contains 1 AGC-kinase C-terminal domain. 
Contains 2 protein kinase domains.

Gene ID:
6195

Database links:

Entrez Gene: 6195 Human

Entrez Gene: 20111 Mouse

Entrez Gene: 81771 Rat

Omim: 601684 Human

SwissProt: Q15418 Human

SwissProt: P18653 Mouse

SwissProt: Q63531 Rat

Unigene: 149957 Human

Unigene: 301827 Mouse

Unigene: 34915 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体 

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hz-1310R Integrin Alpha V + Beta 3 (CD51+CD61)  整合素αVβ3抗体

hz-1356R Integrin Alpha V + Beta 5  整合素αVβ5抗体

hz-5791R Integrin Alpha V + Beta 6  整合素αVβ6抗体

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hz-1109R IQGAP1  支架蛋白抗体

hz-1404R IRF-1/MAR1  干扰素调节因子1抗体

hz-1427R IRAK2  白介素-1受体相关激酶2抗体

hz-3192R Phospho-IRAK1 (Ser376)  磷酸化白介素-1受体相关激酶1抗体

hz-3193R Phospho-IRAK1 (Thr209)  磷酸化白介素-1受体相关激酶1抗体

hz-3194R Phospho-IRAK1 (Thr387)  磷酸化白介素-1受体相关激酶1抗体

hz-2993R IRF3  干扰素调节因子3

hz-3195R Phospho-IRF3 (Ser396)  磷酸化干扰素调节因子3

hz-2440R IRAK4  白介素-1受体相关激酶4抗体

hz-4080R phospho-IRAK4(Thr345)  磷酸化白介素-1受体相关激酶4抗体

hz-2216R IL-1RA  白介素-1受体拮抗剂抗体

hz-2594R IL-1R1/CD121a/IL1 Receptor I  白介素1受体1抗体

hz-5394R phospho-IL-1R1(Tyr496)  磷酸化白介素1受体1抗体

hz-2595R IL-1R2 /CD121b/IL1 Receptor II  白介素1受体2抗体

hz-2382R ST2  白细胞介素1受体相关蛋白抗体

hz-2500R IRF4  干扰素调节因子4抗体

hz-4015R IRGM  免疫相关鸟苷三磷酸酶基因抗体

hz-2634R ILT2/CD85j  细胞表面免疫球蛋白样转录因子2抗体

hz-2994R IRF7  干扰素调节因子7抗体

hz-3196R Phospho-IRF7 (Ser471/472)  磷酸化干扰素调节因子7抗体

hz-0242R IRS P53  胰岛素受体底物p53蛋白抗体

hz-0172R IRS1  胰岛素受体底物-1抗体

hz-2736R phospho-IRS-1(Ser307)  磷酸化胰岛素受体底物p-IRS-1/2抗体

hz-3197R phospho-IRS1(Ser1101)  磷酸化胰岛素受体底物-1抗体

hz-3198R phospho-IRS1(Ser302)  磷酸化胰岛素受体底物-1抗体

hz-3199R phospho-IRS1(Ser332/336)  磷酸化胰岛素受体底物-1抗体

hz-3200R phospho-IRS1(Ser612)  磷酸化胰岛素受体底物-1抗体

hz-3201R phospho-IRS1(Ser636/639)  磷酸化胰岛素受体底物-1抗体

hz-3202R phospho-IRS1(Ser789)  磷酸化胰岛素受体底物-1抗体

hz-3203R phospho-IRS1(Tyr1222)  磷酸化胰岛素受体底物-1抗体

hz-3204R phospho-IRS1(Tyr895)  磷酸化胰岛素受体底物-1抗体

hz-5396R phospho-IRS1(Ser312)  磷酸化胰岛素受体底物-1抗体

hz-5446R phospho-IRS1(Thr176)  磷酸化胰岛素受体底物1抗体

hz-0173R IRS-2  胰岛素受体底物-2抗体

hz-5397R phospho-IRS-2(Ser731)  磷酸化胰岛素受体底物2抗体