磷酸化Rho相关蛋白激酶2抗体

英文名称    phospho-ROCK2(Thr396)    

中文名称    磷酸化Rho相关蛋白激酶2抗体    

别    名    ROCK2(phospho T396); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha.    

磷酸化Rho相关蛋白激酶2抗体         

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    免疫学  信号转导  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, mo,rat,chk,pig,cow,hrs,Rbdog    

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    153kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr396    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化Rho相关蛋白激酶2抗体 产品介绍    background:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.

Subunit:
Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.

Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity. 
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.

Database links:
UniProtKB/Swiss-Prot: O75116.4

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.     

磷酸化Rho相关蛋白激酶2抗体 

hz-3237R Phospho-IKK alpha/beta (Ser176/Ser180)  磷酸化KB抑制蛋白激酶α/β抗体

hz-2513R phospho-NFKBIA(Ser32/36)  磷酸化IKB alpha(Ser32/36)抗体

hz-3233R phospho-IKK beta(Tyr188)  磷酸化KB抑制蛋白激酶β抗体

hz-3231R phospho-IKK beta(Ser466)  磷酸化KB抑制蛋白激酶β抗体

hz-3232R phospho-IKK beta(Tyr199)  磷酸化KB抑制蛋白激酶β抗体

hz-5514R Phospho-NFKBIA (Tyr42)   磷酸化IKB α抗体

hz-5398R phospho-IKK beta(Ser471)  磷酸化KB抑制蛋白激酶β抗体

hz-5399R phospho-IKK beta(Ser740)  磷酸化KB抑制蛋白激酶β抗体

hz-5515R phospho-NFKBIA(Tyr305)  磷酸化IKB alpha抗体

hz-5440R phospho-IKK beta(Ser733)  磷酸化KB抑制蛋白激酶β抗体

hz-5441R phospho-IKK beta(S474)  磷酸化KB抑制蛋白激酶β抗体

hz-5442R phospho-IKK beta(Ser476)  磷酸化KB抑制蛋白激酶β抗体

hz-3227R phospho-IKK gamma (Ser85)  磷酸化KB抑制蛋白激酶γ抗体

hz-3228R phospho-IKK gamma (Ser31)  磷酸化KB抑制蛋白激酶γ抗体

hz-3230R phospho-IKK gamma (Ser376)  磷酸化KB抑制蛋白激酶γ抗体

hz-5393R phospho-IKK gamma(Ser43)  磷酸化KB抑制蛋白激酶γ抗体

hz-4114R IKBKE/IKKi  核因子κB抑制蛋白E抗体

hz-5454R phospho-IKBKE(Thr501)  磷酸化核因子κB抑制蛋白E抗体

hz-5517R phospho-NFKBIE (Ser157)  磷酸化KB抑制蛋白激酶ε

hz-0245R IKI3 family protein  拟南芥IKI3抗体

hz-2428R IL-9  白介素9抗体

hz-0698R IL-10  白细胞介素-10抗体

hz-1827R IL-11  白介素11抗体

hz-2459R IL-10RA  白细胞介素-10受体a抗体

hz-2602R IL-10RB/CRFB4  白细胞介素-10受体β抗体

hz-0767R IL-12  白介素12抗体

hz-1789R IL-12  白介素12抗体

hz-2603R IL-12RB1/CD212  白细胞介素-12受体β1抗体

hz-2604R IL12RB2  白细胞介素-12受体β2抗体

hz-0560R IL-13  白介素13抗体

hz-1771R IL-14/Taxilin alpha  白介素14抗体

hz-2605R IL-15RA  白介素15受体α抗体

hz-1829R IL-15  白介素15抗体

hz-1830R IL-16  白介素16抗体

hz-2460R IL-13Ra1  白细胞介素-13受体a1抗体

hz-2461R IL-13Ra2  白细胞介素-13受体a2抗体

hz-0768R IL-16/LCF  白介素-16抗体

hz-1183R IL-17A  白介素-17抗体

hz-2140R IL-17  白介素-17抗体

hz-2606R IL-17R/IL-17RA/CD217  白介素17受体抗体

hz-2607R IL-17RC/IL-17RL  白介素17受体C抗体

hz-2608R IL-17RD  白介素17受体D抗体

hz-2609R IL-17B  白介素-17B抗体