磷酸化细胞迁移诱导因子5抗体

英文名称    phospho-RAC1 + RAC2 (Ser71)    

中文名称    磷酸化细胞迁移诱导因子5抗体    

别    名    RAC1(phospho S71); RAC2(phospho S71); RAC1 + RAC2 (phospho Ser71); Cell migration inducing gene 5 protein; Cell migration-inducing gene 5 protein; MIG5; MIG 5; Migration inducing gene 5; Migration inducing protein 5; p21 Rac1; Rac 1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1; Rho family small GTP binding protein Rac1; TC 25; TC25; MGC111543; p21-Rac1; RAC1; RAC1_HUMAN; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; rho family small GTP binding protein Rac1.    

磷酸化细胞迁移诱导因子5抗体     

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  免疫学  信号转导  G蛋白偶联受体      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    22kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human RAC1 around the phosphorylation site of Ser71    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化细胞迁移诱导因子5抗体产品介绍    background:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Function:
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. 
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Subunit:
Interacts with NISCH. Interacts with PIP5K1A. Interacts with the GTP-bound form of RAB7A. Interacts with SRGAP2. Interacts with CYFIP1/SRA-1. Interacts with PLXNB3. Interacts with ARHGDIA; the interaction is induced by SEMA5A, mediated through PLXNB3 and inactivates and stabilizes RAC1. Interacts (GTP-bound form preferentially) with PKN2 (via the REM repeats); the interaction stimulates autophosphorylation and phosphorylation of PKN2. Interacts with the GEF proteins PREX1, RASGRF2, FARP1, FARP2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM; the interaction requires PAK1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with TBC1D2. Interacts with UNKL. Interacts with USP6. Interacts with SPATA13. Interacts with ARHGEF16; mediates activation of RAC1 by EPHA2. Interacts with ITGB4. Interacts with S100A8 and calprotectin (S100A8/9). Interacts with PACSIN2. Interacts with ITGB1BP1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts.

Tissue Specificity:
Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Post-translational modifications:
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. 
GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome.

Similarity:
Belongs to the small GTPase superfamily. Rho family.

Gene ID:
5879

Database links:

Entrez Gene: 5879 Human

Entrez Gene: 5880 Human

Entrez Gene: 19353 Mouse

Entrez Gene: 19354 Mouse

Entrez Gene: 363875 Rat

Entrez Gene: 366957 Rat

Omim: 602048 Human

Omim: 602049 Human

SwissProt: P15153 Human

SwissProt: P63000 Human

SwissProt: P63001 Mouse

SwissProt: Q05144 Mouse

SwissProt: Q6RUV5 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化细胞迁移诱导因子5抗体

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hz-1770R FSP/Spastin  纤维母细胞表面蛋白抗体

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hz-1769R FHIT  脆性组氨酸三联体抗体

hz-3146R Phospho-FHIT (Tyr114)  磷酸化脆性组氨酸三联体抗体

hz-4044R Glucose 6 phosphatase alpha  葡萄糖6磷酸酶α抗体

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hz-3942R G protein beta 2/GNB2  G蛋白β2抗体/鸟嘌呤核苷酸结合蛋白β亚基2

hz-3943R G protein beta 3/GNB3  G蛋白β3抗体/鸟嘌呤核苷酸结合蛋白β亚基3

hz-3940R G protein beta 4/GNB4  G蛋白β4抗体/鸟嘌呤核苷酸结合蛋白β亚基4

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