磷酸化复制因子A蛋白2抗体

英文名称    phospho-RPA2(Thr21)    

中文名称    磷酸化复制因子A蛋白2抗体    

别    名    RPA32/RPA2(phospho T21); 60S acidic ribosomal protein P1; p32; p34; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2, 32kDa; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32; RPA34; RpLP1; RpP2.    

磷酸化复制因子A蛋白2抗体       

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    免疫学  染色质和核信号      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    29 kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化复制因子A蛋白2抗体产品介绍    background:
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Function:
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. 
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. 

Subunit:
Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 

Subcellular Location:
Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.

Post-translational modifications:
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as a hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence. 

Database links:
UniProtKB/Swiss-Prot: P15927.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化复制因子A蛋白2抗体

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hz-2351R E. coli LPS  大肠杆菌脂多糖抗体

hz-1876R EP3  前列腺素E受体3抗体

hz-5884R Endomucin  内皮粘蛋白hzCN抗体

hz-1763R EphA1/Ephrina1/Ephrin A1 Receptor  内皮细胞受体蛋白酪氨酸激酶A抗体（肿瘤坏死因子α诱导蛋白4）

hz-1764R EphA4  酪氨酸蛋白激酶A4抗体

hz-2764R Ephrin B  Ephrin B抗体

hz-3217R phospho-Ephrin B(Tyr329)  磷酸化Ephrin B抗体

hz-3167R phospho-Ephrin B(Tyr316)  磷酸化Ephrin B抗体

hz-3128R phospho-Ephrin B(Ser300)  磷酸化Ephrin B抗体

hz-3129R phospho-Ephrin B(Tyr324/329)  磷酸化Ephrin B抗体

hz-6047R Ephrin A3/EFNA3  酪氨酸蛋白激酶A3受体抗体

hz-6048R Ephrin A5  酪氨酸蛋白激酶A5受体抗体

hz-5887R HPSE2  乙酰肝素酶样蛋白抗体

hz-0485R EphA2/Eph receptor A2  内皮细胞受体蛋白酪氨酸激酶抗体

hz-3126R Phospho-EphA2(Tyr594)  磷酸化酪氨酸蛋白激酶A2受体抗体

hz-1455R EphA4 R/Eph receptor A4  酪氨酸蛋白激酶A4受体抗体

hz-0247R EphB2 R  酪氨酸蛋白激酶受体B2抗体

hz-2343R EPO  红细胞生成素抗体

hz-1424R EPOR  红细胞生成素受体抗体

hz-3880R EPHX2  胞浆环氧化物水解酶抗体

hz-2365R Eppin  附睾蛋白酶抑制蛋白

hz-3881R EPX  嗜酸性粒细胞过氧化物酶抗体

hz-2571R EphB6  酪氨酸蛋白激酶受体B6抗体

hz-3848R EPS8  表皮生长因子受体底物8抗体

hz-0725R ERα/ESR1  雌激素受体α抗体

hz-0021M ERAB/HSD17B10  内质网Aβ相关结合蛋白抗体

hz-5890R Lumican  基膜聚糖蛋白抗体

hz-1726R ERCC1  DNA切除修复蛋白1抗体

hz-0022R ERK2/MAPK1  丝裂原活化蛋白激酶1抗体

hz-1736R phospho-MEK1/MAPKK1(Ser298)  磷酸化丝裂原活化蛋白激酶激酶1抗体

hz-3269R phospho-MEK1(Thr286)  磷酸化丝裂原活化蛋白激酶激酶1抗体

hz-3270R Phospho-MEK1/2(Ser218/222)  磷酸化丝裂原活化蛋白激酶激酶1/2抗体

hz-3722R Phospho-MEK1/2(Ser222)  磷酸化丝裂原活化蛋白激酶激酶1/2抗体

hz-1522R phospho-ERK1/MAPK-1/2(Thr183/185)  磷酸化丝裂原活化蛋白激酶1/2抗体

hz-5411R phospho-MEK1/MAP2K1(Ser385)   磷酸化丝裂原活化蛋白激酶1抗体

hz-5412R phospho-MEK1/MAP2K1(Thr292)  磷酸化丝裂原活化蛋白激酶1抗体

hz-1645R phospho-ERK1(Thr202/Tyr204)  磷酸化丝裂原活化蛋白激酶1抗体

hz-5413R phospho-MEK1/MAP2K1(Thr386)  磷酸化丝裂原活化蛋白激酶1抗体

hz-5414R phospho-MEK1+MEK2(Ser222/Ser226)  磷酸化丝裂原活化蛋白激酶1抗体