英文名称 RNA polymerase II CTD repeat YSPTSPS (phospho S2)
中文名称 磷酸化RNA聚合酶II CTD抗体
别 名 Rpb1 CTD; RNA polymerase II; RNAPII;POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
磷酸化RNA聚合酶II CTD抗体
说 明 书 0.1ml
产品类型 磷酸化抗体
研究领域 细胞生物 免疫学 信号转导 转录调节因子 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow,
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 217kDa
细胞定位 细胞核
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II RPB1 around Y(p-S)PTSPS
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
磷酸化RNA聚合酶II CTD抗体 产品介绍 background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Subunit:
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity). Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact.
Subcellular Location:
Nucleus.
Post-translational modifications:
Dephosphorylated by the protein phosphatase CTDSP1.
Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity).
Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs.
Similarity:
Belongs to the RNA polymerase beta' chain family.
Database links:
UniProtKB/Swiss-Prot: P24928.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化RNA聚合酶II CTD抗体
hz-3118R Phospho-DARPP32(Thr34) 磷酸化多巴胺/cAMP调节神经磷蛋白抗体
hz-3119R Phospho-DARPP32(Thr75) 磷酸化多巴胺/cAMP调节磷蛋白抗体
hz-2332R DGAT1/Diglyceride acyltransferase 二脂酰甘油酰基转移酶抗体
hz-1007R DRD1 多巴胺受体D1抗体
hz-5825R PCDHA12 原钙粘蛋白12抗体
hz-5826R PCDHAC1 原钙粘蛋白1抗体
hz-1008R DRD2 多巴胺受体D2抗体
hz-1743R DRD3 多巴胺受体D3抗体
hz-1714R DAT/Dopamine transporter 多巴胺转运蛋白DAT抗体
hz-1746R DRD4 多巴胺受体D4抗体
hz-1747R DRD5/Dopamine receptor 5 多巴胺受体D5抗体
hz-2761R DBC1/deleted in bladder cancer 1 膀胱癌缺失基因1
hz-3117R Phospho-DBC1 (Thr454) 磷酸化膀胱癌缺失基因1抗体
hz-0596R DBH 多巴胺β羟化酶抗体
hz-5827R PCDHGB5 原钙粘蛋白γB5抗体
hz-3674R PBR/DBI/TSPO 外周苯二氮受体抗体
hz-0592R DCC 结肠直肠癌缺失基因抗体
hz-2564R DCIR/CLEC4A C型凝集素结构域家族4成员A抗体
hz-1695R DCN/Decorin 核心蛋白聚糖抗体
hz-2663R CLEC5A/MDL1 C型凝集素结构域家族5成员A抗体
hz-2556R LAMP-3/DC-LAMP/CD208 溶酶体相关膜蛋白3抗体
hz-2557R DC-SIGN/CD209 细胞间粘附分子非整合素蛋白3抗体
hz-2239R DC-SIGN/CD209 细胞间粘附分子非整合素蛋白3抗体
hz-2563R DC-SIGNR/CD299 CD299抗体
hz-2565R DEC-205/CD205/Ly75 淋巴细胞抗原75抗体
hz-5056R DECR1 线粒体2,4-双烯酰辅酶A还原酶1抗体
hz-5843R PCDHB11 原钙粘蛋白β11抗体
hz-4057R DEGS1 退行性精母细胞同源物1抗体
hz-1037R DCP 异常凝血酶原/脱-γ-羧基凝血酶原抗体
hz-5828R PCDHB13 原钙粘蛋白13抗体
hz-5057R DHCR7 7脱氢胆固醇还原酶抗体
hz-1269R Doublecortin 双皮质素抗体
hz-3113R Phospho-Doublecortin (Ser297) 磷酸化双皮质素抗体
hz-2083R DcR1 肿瘤坏死因子配体超家族成员10C
hz-1694R DcR2/CD264/TNFRSF10D 诱捕受体2抗体
hz-2493R TNFRSF12A/TWEAKR/CD266 肿瘤坏死因子配体超家族成员12A抗体
hz-1716R DcR3/TR6 诱捕受体3抗体
hz-2785R DDAH-II 双甲基精氨酸水解酶2抗体
hz-3764R DDX19B/DBP5 解旋酶DEAD5抗体
hz-3514R D-DIMER 交联纤维蛋白二聚体抗体
hz-0993R DDX58 DDX58抗体
hz-5299R phospho-DDX58 (Ser8) 磷酸化DDX58抗体
hz-5300R phospho-DDX58 (Thr170) 磷酸化DDX58抗体
hz-2588R DDB1 DNA损伤结合蛋白1抗体
hz-2599R DDB2 DNA损伤结合蛋白2抗体
hz-0180R DDC/DOPA Decarboxylase 多巴胺脱羧酶抗体
hz-5829R NG2 硫酸软骨素蛋白多糖4/黑色素瘤相关蛋白抗体
hz-3597R DDX4/MVH DDX4抗体
hz-1697R DEF1/α Defensin 1/3 防御素α1/3抗体
hz-2799R Defensin alpha 4 防御素α4抗体
hz-1163R Defensin β1 防御素β1抗体
hz-5830R RAB21 G蛋白家族RAB21蛋白抗体
关注
已认证
