Rho GTP酶激活蛋白GAP抗体

英文名称    RACGAP1    

中文名称    Rho GTP酶激活蛋白GAP抗体    

别    名    GAP; Gap1; GTPase activating protein; HsCYK 4; HsCYK4; ID GAP; KIAA1478; MgcRacGAP; Rac GTPase activating protein 1; RACGAP 1; RGAP1_HUMAN.    

Rho GTP酶激活蛋白GAP抗体      

说 明 书    0.2ml      

研究领域    细胞生物  信号转导  转录调节因子  细胞分化  G蛋白信号      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Cow,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    71kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RACGAP1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

Rho GTP酶激活蛋白GAP抗体 产品介绍    background:
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Function:
Component of the centralspindlin complex that serves asa microtubule-dependent and Rho-mediated signaling required for themyosin contractile ring formation during the cell cyclecytokinesis. Plays key roles in controlling cell growth anddifferentiation of hematopoietic cells through mechanisms otherthan regulating Rac GTPase activity. Also involved in theregulation of growth-related processes in adipocytes and myoblasts.May be involved in regulating spermatogenesis and in the RACGAP1pathway in neuronal proliferation. Shows strong GAP (GTPaseactivation) activity towards CDC42 and RAC1 and less towards RHOA.Essential for the early stages of embryogenesis. May play a role inregulating cortical activity through RHOA during cytokinesis. Mayparticipate in the regulation of sulfate transport in male germcells.

Subunit:
Heterotetramer of two molecules each of RACGAP1 andKIF23. Found in the centralspindlin complex composed of RACGAP1 andKIF23. Associates with alpha-, beta- and gamma-tubulin andmicrotubules. Interacts via its Rho-GAP domain with RND2.Associates with AURKB during M phase. Interacts via its Rho-GAPdomain and basic region with PRC1. The interaction with PRC1inhibits its GAP activity towards CDC42 in vitro, which may berequired for maintaining normal spindle morphology. Interacts withSLC26A8 via its N-terminus. Interacts with RAB11FIP3. Interactswith ECT2; the interaction is direct, occurs at anaphase and duringcytokinesis in a microtubule-dependent manner and is enhanced byphosphorylation by PLK1. Interacts with KIF23; the interaction isdirect.

Subcellular Location:
ucleus. Cytoplasm. Cytoplasm, cytoskeleton,spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavagefurrow. Midbody. Note=Colocalizes with RND2 in Golgi-derivedproacrosomal vesicles and the acrosome (By similarity). Duringinterphase, localized to the nucleus and cytoplasm along withmicrotubules, in anaphase, is redistributed to the central spindleand, in telophase and cytokinesis, to the midbody. Colocalizes withRHOA at the myosin contractile ring during cytokinesis. Colocalizeswith ECT2 to the mitotic spindles during anaphase/metaphase, thecleavage furrow during telophase and at the midbody at the end ofcytokinesis. Colocalizes wit Cdc42 to spindle microtubules fromprometaphase to telophase.

Tissue Specificity:
Highly expressed in testis, thymus andplacenta. Expressed at lower levels in spleen and peripheral bloodlymphocytes. In testis, expression is restricted to germ cells withthe highest levels of expression found in spermatocytes. Expressionis regulated in a cell cycle-dependent manner and peaks during G2/Mphase.

Post-translational modifications:
Phosphorylated at multiple sites in the midbody duringcytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is,at least in part, responsible for exerting its latent GAP activitytowards RhoA. Phosphorylation on multiple serine residues by PLK1enhances its association with ECT2 and is critical for cleavagefurrow formation.

Similarity:
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain.

Database links:
UniProtKB/Swiss-Prot: Q9H0H5.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

Rho GTP酶激活蛋白GAP抗体 

hz-2251R A Raf/ARAF  A-Raf抗体

hz-3005R phospho-A Raf(Ser214)  磷酸化A-Raf抗体

hz-3371R Phospho-A Raf(Ser299)  磷酸化A-Raf抗体

hz-3421R Phospho-A Raf(Tyr301/302)  磷酸化A-Raf抗体

hz-1089R B-Raf  B Raf抗体

hz-1703R c-Raf/Raf1  癌基因c-Raf抗体

hz-3013R phospho-B-Raf (Ser365)  磷酸化B-Raf抗体

hz-3373R phospho-B-Raf (Ser444)  磷酸化B-Raf抗体

hz-3374R phospho-B-Raf (Ser602)  磷酸化B-Raf抗体

hz-5232R phospho-B-Raf (Ser446)  磷酸化B-Raf抗体

hz-5233R phospho-B-Raf (Thr597)  磷酸化B-Raf抗体

hz-5224R phospho-B-Raf (Thr401)  磷酸化B-Raf抗体

hz-0802R BRCA1/BAP1  乳腺癌易感基因1抗体

hz-3051R Phospho-BAP1(Ser592)  磷酸化乳腺癌易感基因1抗体(人)

hz-3014R Phospho-BRCA1(Ser1524)  磷酸化乳腺癌易感基因1抗体

hz-5710R BRCC4/BRCC45  乳腺癌易感基因复合蛋白4抗体

hz-5221R Phospho-BRCA1(Ser1189)  磷酸化乳腺癌易感基因1抗体

hz-5222R Phospho-BRCA1(Ser1423)  磷酸化乳腺癌易感基因1抗体

hz-0803R BRCA1/BAP1  乳腺癌易感基因1抗体

hz-1210R BRCA2  乳腺癌易感基因2抗体

hz-0489R BrdU  溴脱氧尿苷抗体

hzm-0917M BrdU(A7)  溴脱氧尿苷单克隆抗体

hz-0917R BrdU  溴脱氧尿苷抗体

hz-3669R BRN3A  转录因子Brn3蛋白抗体Brn3α

hz-3668R Brs3/Bombesin Receptor 3  蛙皮素受体3抗体

hz-1408R SMARCA4/SNF2 beta/BRG1  抑癌基因SNF2β抗体

hz-1015R BRMS-1  乳腺癌转移抑制基因1抗体

hz-2229R Brucella  布氏杆菌菌体蛋白抗体

hz-0292R BSA/Bovine Serum Albumin  牛血清白蛋白抗体

hz-1954R BSEP/ABCB11  胆汁酸盐输出泵蛋白抗体

hz-2668R Bone sialoprotein  骨涎蛋白抗体

hz-0031R BTG2/TIS21  B细胞迁移基因2抗体

hz-2752R BTK/ATK  蛋白酪氨酸激酶ATK抗体

hz-3055R Phospho-Btk(Ser180)  磷酸化蛋白酪氨酸激酶ATK抗体

hz-3069R Phospho-BTK/BPK(Tyr223)  磷酸化蛋白酪氨酸激酶ATK抗体

hz-3820R BAP135/TFII I  蛋白酪氨酸激酶BAP135抗体

hz-4179R RAD9  细胞周期检查控制蛋白质抗体

hz-5642R phospho-RAD9(Ser272)  磷酸化细胞周期检查控制蛋白质抗体