环指蛋白75抗体

英文名称    RNF75    

中文名称    环指蛋白75抗体    

别    名    E3 ubiquitin-protein ligase RBX1; MGC13357; MGC1481; Protein ZYP; Rbx 1; Rbx1; RBX1_HUMAN; Regulator of Cullins 1; Ring box 1; RING box protein 1; RING finger protein 75; RING finger protein; RING-box protein 1; Ringbox protein 1; RNF 75; RNF75; ROC 1; ZYP protein.    

环指蛋白75抗体      

说 明 书    0.1ml  0.2ml      

研究领域    细胞生物  免疫学  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Cow,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    12kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ROC1/RNF75    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

环指蛋白75抗体产品介绍    background:
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.

Function:
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. 

Subunit:
Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5 and VHL. Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Part of multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B. Interacts with UBE2M. Part of a SCF complex consisting of CUL1, FBXO3, RBX1 and SKP1; this complex interacts with PML via FBXO3. Interacts with human adenovirus early E1A protein; this interaction inhibits RBX1-CUL1-dependent elongation reaction of ubiquitin chains by the SCF(FBW7) complex. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and CDC34. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein. Part of the BCR(ENC1) complex containing ENC1. Part of the BCR(GAN) complex containing GAN. Part of the BCR(KLHL41) complex containing KLHL41. Part of the BCR(KEAP1) complex containing KEAP1. 

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the RING-box family. 
Contains 1 RING-type zinc finger.

Database links:
UniProtKB/Swiss-Prot: P62877.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

环指蛋白75抗体

hz-8565R RENT1/hUPF1  ATP依赖解旋酶RENT1抗体

hz-8566R Phospho-NMDAR2B (Tyr1474)  磷酸化谷氨酸受体2B抗体

hz-8567R Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252)  磷酸化谷氨酸受体2A+2B抗体

hz-8568R Wnt11  信号通路Wnt11抗体

hz-8569R TFE3  转录因子E3

hz-8570R NGN1/Neurogenin 1  神经元素1抗体

hz-8571R Ubiquityl Histone H2A.X (Lys119)  泛素化组蛋白H2AX抗体

hz-8572R phospho-CPLA2(Ser505)  磷酸化胞浆型磷脂酶A2抗体

hz-8573R DHRS2  脱氢酶/还原酶家族成员2抗体

hz-8574R ox-LDL  氧化低密度脂蛋白抗体

hz-8575R FSIP1  纤维性鞘结合蛋白1抗体

hz-8576R GNRPX/PLEKHJ1  鸟嘌呤核苷酸结合蛋白X抗体

hz-8577R GPKOW  G蛋白修补结构域蛋白5抗体

hz-8578R GRAMD2  GRAM结构域蛋白2抗体

hz-8579R PMS1  肿瘤错配修复基因PMS1抗体

hz-8580R GRAMD3/NS3TP2  丙型肝炎病毒-NS3反转录激活蛋白2抗体

hz-4168R TNIK  TRAF2和NCK激酶相互作用蛋白抗体

hz-8581R GSTCD  谷胱甘肽硫转移酶C段结构域抗体

hz-8582R GTDC1  糖基转移酶样1抗体

hz-8583R phospho-IKKi/IKKe(Ser172)  磷酸化核因子NFκB抑制蛋白激酶i抗体

hz-8584R PXMP1/ABCD3  过氧化物酶膜蛋白1抗体

hz-5598R phospho-TNIK(Ser764)  磷酸化TRAF2和NCK激酶相互作用蛋白抗体

hz-8585R Arginase 1  精氨酸酶1抗体

hz-8586R GIPC-2  胞浆区相关蛋白GIPC2抗体

hz-8587R DOK5/IRS6  胞浆接头蛋白Dok5抗体

hz-8588R NETO1  脑低密度脂蛋白受体蛋白1抗体

hz-8589R Transglutaminase 2/TGase2  转谷氨酰胺酶2抗体

hz-8590R RanBP7/Importin 7  RAN结合蛋白7抗体

hz-5599R phospho-TNIK(Ser769)   磷酸化TRAF2和NCK激酶相互作用蛋白抗体

hz-8591R IL-9  白介素9抗体

hz-8592R ERM/Etv5  转录因子Ets差异基因5抗体

hz-8593R UGCG/Ceramide glucosyltransferase  葡萄糖神经酰胺合成酶抗体

hz-8594R ACTBL1/Ovary  卵巢、胎盘、前列腺、睾丸蛋白22抗体

hz-8595R C9orf72  9号染色体开放阅读框72抗体

hz-8599R TAS2R7/T2R6  味觉感受器蛋白T2R6抗体

hz-1171R Annexin I  膜粘连蛋白 I抗体

hz-8612R GPR70/T1R1  蛋白偶联受体70抗体

hz-4176R SLC29A4  脑质膜单胺转运蛋白PMAT抗体

hz-5637R phospho-SLC29A4(Tyr198)  磷酸化脑质膜单胺转运蛋白PMAT抗体

hz-8629R phospho-Na+/K+-ATPase Alpha1(Ser 943)  磷酸化钠钾ATP酶α1多肽抗体

hz-8634R FOXA1/HNF3-alpha  转录因子HNF-3α抗体

hz-8650R T2R38/TAS2R38  味觉感受器蛋白T2R38抗体

hz-4156R PRKD1/PKC mu  蛋白激酶C mu型抗体

hz-8654R FAM81A  FAM81A蛋白抗体