DNA修复蛋白Rad50抗体

英文名称    RAD50    

中文名称    DNA修复蛋白Rad50抗体    

别    名    DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein.    

 DNA修复蛋白Rad50抗体      

说 明 书    0.2ml      

研究领域    染色质和核信号  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    154kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RAD50 (817-872aa)    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

 DNA修复蛋白Rad50抗体产品介绍    background:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Function:
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Subunit:
Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. 

Subcellular Location:
Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.

Tissue Specificity:
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR 

DISEASE:
Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.

Similarity:
Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain.

Database links:
UniProtKB/Swiss-Prot: Q92878.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

 DNA修复蛋白Rad50抗体

hz-1011 Fc GammaR I /CD64(Human Receptor I for the Fc region of immunoglobulin G,Fc GammaR I ) ELISA Kit  人免疫球蛋白G Fc段受体Ⅰ

EF-1011 C3 ELISA Kit  鲑鱼补体蛋白3

EA-1011 Alpha2-AP (Rabbit Alpha2-Antiplasmin) ELISA Kit  兔子α2抗纤溶酶

A-43 RV IgM  风疹胞病毒 IgM(捕获法二步法)

A-16-1 TOXO IgG  弓形虫抗体 IgG(间接法二步法)

A-15-1 TOXO IgM  弓形虫抗体 IgM(间接法二步法)

A-33 TOXO IgM  弓形虫抗体 IgM(捕获法一步法)

A-44 TOXO IgM  弓形虫抗体 IgM(捕获法二步法)

A-40-1 HSV- I IgG  单纯疱疹Ⅰ型病毒 IgG(间接法二步法)

A-18-1 HSV- I IgM  单纯疱疹Ⅰ型病毒 IgM(间接法二步法)

A-36 HSV- I IgM  单纯疱疹Ⅰ型病毒 IgM（捕获法一步法）

A-45 HSV- I IgM  单纯疱疹Ⅰ型病毒 IgM（捕获法二步法）

A-41-1 HSV- II IgG  单纯疱疹Ⅱ型病毒 IgG(间接法二步法)

A-19-1 HSV- II IgM  单纯疱疹Ⅱ型病毒 IgM(间接法二步法)

A-37 HSV- II IgM  单纯疱疹Ⅱ型病毒 IgM （捕获法，一步法）

A-46 HSV- II IgM  单纯疱疹Ⅱ型病毒 IgM （捕获法，二步法）

E-73-48/96 Anti-Ins  胰岛素抗体（间接法二步法）

E-81-48/96 Anti-GAD  谷氨酸脱羧酶抗体（间接法二步法）

E-82-48/96 Ins  胰岛素（间接法二步法）

E-83-48/96 C-Peptide  C-肽（夹心法二步法）

E-91-48/96  破伤风抗体（双抗原夹心法）

A-31  包虫抗体IgG（间接法二步法）

hz-1012 DAO(Rat diamine oxidase,DAO) ELISA Kit  大鼠二胺氧化酶

hz-1012 Mouse anti-red cell antibody ELISA Kit   小鼠抗红细胞抗体

hz-1012 GCP-2/CXCL6(Human granulocyte chhzotactic protein-2,GCP-2) ELISA Kit  人粒细胞趋化蛋白-2

EA-1012 SAA (Rabbit Serum amyloid A) ELISA Kit  兔血清淀粉样蛋白A

EF-1012 C4 ELISA Kit   鲑鱼补体蛋白4

A-06-1 MV IgG  麻疹病毒 IgG（间接法二步法）

A-05-1 MV IgM  麻疹病毒 IgM（间接法二步法）

A-47-1 COX IgG  柯萨奇病毒 IgG 混合型（间接法二步法）

A-07-1 COX IgM  柯萨奇病毒 IgM 混合型(间接法二步法)

A-48-1 COX IgG I  柯萨奇病毒 IgG Ⅰ(间接法二步法)

A-08-1 COX IgM I  柯萨奇病毒 IgM Ⅰ(间接法二步法)

A-49-1 COX IgG II  柯萨奇病毒 IgG Ⅱ(间接法二步法)

A-50-1 COX IgG III  柯萨奇病毒 IgG Ⅲ(间接法二步法)

A-51-1 COX IgG IV  柯萨奇病毒 IgG Ⅳ(间接法二步法)

A-52-1 COX IgG V  柯萨奇病毒 IgG Ⅴ