环指蛋白146抗体

英文名称    RNF146    

中文名称    环指蛋白146抗体    

别    名    Dactylidin; dJ351K20.1; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1.    

环指蛋白146抗体        

说 明 书    0.1ml  0.2ml      

研究领域    环指蛋白      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    39kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RNF146 (95-160aa)    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

环指蛋白146抗体产品介绍    background:
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF146 (RING finger protein 146), also known as Dactylidin, is a 359 amino acid protein that contains one RING-type zinc finger and one WWE domain. Via its RING-type zinc finger, RNF146 may play a role in transcriptional regulation and protein degradation events. Defects in the gene encoding RNF146 are associated with Alzheimer's disease (AD) and may lead to a higher risk of breast cancer. Two isoforms of RNF146 exist due to alternative splicing events.

Function:
E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.

Subunit:
Can form homooligomers. Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, HIST1H1C, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6. Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding. 

Tissue Specificity:
Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.

Post-translational modifications:
Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.

DISEASE:
Note=Defects in RNF146 are a cause of susceptibility to breast cancer.

Similarity:
Contains 1 RING-type zinc finger.
Contains 1 WWE domain.

Database links:

Entrez Gene: 81847 Human

Omim: 612137 Human

SwissProt: Q9NTX7 Human

Unigene: 267120 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

环指蛋白146抗体

hz-1298P Smac/DIABLO(the second mitochondrial activator of caspase)  线粒体促凋亡蛋白抗原

hz-0699P SLC33A1(Acetyl-coenzyme A transportor 1)  乙酰辅酶A转运蛋白1抗原

hz-0856P SLC34A2/NaPi-2b(Solute carrier family 34 mhzber 2)  磷酸钠协同转运蛋白抗原

hz-2034P SMN1(survival of motor neuron 1)  运动神经元生存蛋白1抗原

hz-2224P phospho-Smad2(p-Ser465)petide  磷酸化Smad2抗原

hz-0585P Smad4(Mothers against decapentaplegic homolog 4)  Smad4抗原

hz-0566P Smad7(Mothers against decapentaplegic homolog 7)  Smad 7(多肽)

hz-0457P phosphor-SMAD(p-Ser425)、phosphor-Mothers against decapentaplegic  磷酸化SMAD(p-Ser425)抗原

hz-1131P SNAP-25 (synaptosomal-associated protein 25)  突触相关膜蛋白25抗原

hz-1371P Snail  Snail蛋白抗原

hz-0580P Socs 3 (suppressor of cytokine signaling 3)  细胞因子信号传导抑制蛋白3抗原

hz-1079P SOD1(superoxide-dimutase-1)  超氧化物歧化酶1抗原

hz-1080P SOD2 (superoxide-dimutase-2)  超氧化物歧化酶2抗原

hz-1132P Somatostatin/GRIH  生长抑素抗原

hz-0523P Sox2  胚胎干细胞关键蛋白

hz-0975P TSFP1/SP1(transcription factor Sp1)  SP1转录生长因子抗原

hz-1133P SPARC (secreted protein acidic and rich in cysteine)  富含半胱氨酸的酸性分泌蛋白抗原

hz-1401P SRF(Serum response factor)  血清应答因子抗原

hz-1402P SREBP-1(Sterol Regulatory Elhzent Binding Protein-1)  固醇调节元件结合蛋白1抗原

hz-1381P AKAP12A/SSeCKS peptide  丝氨酸抑制蛋白激酶C底物抗原

hz-1702P Fut4/CD15/SSEA-1(Stage-specific hzbryonic surface antigen 1)  阶段特异性胚胎表面抗原-1抗原

hz-1137P SSTR1 (somatostatin receptor 1)  生长抑素受体1(SSTR1)抗原

hz-1138P SSTR2 (somatostatin receptor 2)  生长抑素受体2抗原

hz-1368P SSTR3 peptide  生长抑素受体3抗原

hz-1139P SSTR5 (somatostatin receptor 5)  生长抑素受体5抗原

hz-1317P STAT1(signal transducers and activators of transcription 1)  信号转导与转录激活因子1抗原

hz-1657P phospho-STAT1(p-Tyr701) peptide  磷酸化信号转导与转录激活因子1抗原

hz-1141P STAT3(signal transducers and activators of transduction3)  信号转导和转录激活因子3抗原

hz-1142P STAT5(signal transducers and activators of transduction5)  信号转导和转录激活因子5(STAT5)抗原

hz-1143P STAT6(signal transducers and activators of transduction 6)  信号转导和转录激活因子6抗原

hz-0629P SULT1E1(Estrogen Sulfotranferase)  雌激素硫酸转移酶抗原

hz-1499P SUMO  类泛素蛋白

hz-0622P Gamma-Synuclein/SNCG  核突触蛋白-γ抗原

hz-0885P phospho-Tau protein (pThr489) peptide  磷酸化微管相关蛋白多肽

hz-1369P MAP-2(microtubule associated protein 2)  微管相关蛋白2抗原

hz-1847P MAP1A/Mtap1a(microtubule associated protein 1A)  微管相关蛋白1A抗原

hz-1104P TBX2/T-box2(T-box Protein 2)  新型抑癌基因(多肽)

hz-1280P TCF7L2(transcription factor 7-like 2)  转录因子7类似物2抗原

hz-0822P TDP-43/TARDBP (tar DNA binding protein)  Tar DNA 结合蛋白43抗原

hz-1039P Tenascin  固生蛋白抗原