受体相互作用蛋白激酶1抗体

英文名称    RIP1    

中文名称    受体相互作用蛋白激酶1抗体    

别    名    RLIP1; RalA-binding protein 1; 76-kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; Ral-interacting protein 1; RalA-binding protein 1; RalBP1; 76 kDa Ral-interacting protein; DNP-SG ATPase; RalA-binding protein 1; RBP1_HUMAN; RLIP1; RLIP76.    

受体相互作用蛋白激酶1抗体       

说 明 书    0.1ml  0.2ml      

研究领域    细胞生物  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Chicken, Pig,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    74kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RIP1/RalBP1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

受体相互作用蛋白激酶1抗体产品介绍    background:
Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.

Function:
Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex.

Subunit:
Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK.

Subcellular Location:
Cytoplasm. Cell membrane.

Tissue Specificity:
Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. 

Post-translational modifications:
Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction. 
RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-161 by RIPK3 is necessary for the formation of the necroptosis-inducing complex. 
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently downregulates TNF-alpha-induced NFkappa-B signaling. Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.

Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain. 
Contains 1 protein kinase domain.

Database links:
UniProtKB/Swiss-Prot: Q13546.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.     

受体相互作用蛋白激酶1抗体

hz-1084P PEG10(Paternally expressed gene 10)  肝癌高表达基因抗原

hz-2106P PENK/Enkephalin A(Preproencephalin A)  脑啡肽

hz-0584P PEPT2 (Peptide-transporters 2)  肠道肽转运蛋白2/小肽转运蛋白2抗原

hz-2469P PERK(PRKR-like endoplasmic reticulum kinase)  蛋白激酶R样内质网激酶抗原

hz-0796P FSCN1 (Fascin 1 protein; fascin homolog 1, actin-bundling protein)  纤维束蛋白同源物1/肌动蛋白集束蛋白/圆线虫紫癜 抗原

hz-0563P P-GP(P-glycoprotein)  P-糖蛋白抗原

hz-1551P Pepsinogen C peptide  胃蛋白酶原C蛋白抗原

hz-1832P PGC-1 Alpha(peroxisome proliferator-activated receptor- Gamma coactivator 1 Alpha)  过氧化物酶体增殖物激活受体γ辅激活子1α抗原

hz-0823P PGRN (progranulin)  颗粒蛋白前体抗原

hz-1449P PHB(Prohibitin)  抑制素/肿瘤抑制因子抗原

hz-0520P PKA(Protein Kinase A)  蛋白激酶A(多肽)

hz-1098P PLAU/uPA (Plasminogen activator,urokinase)  尿激酶型纤溶酶原激活因子（多肽）

hz-1048P Podoplanin Protein  平足蛋白抗原

hz-0891P Pokhzon (POK Erythroid Myeloid Ontogenic factor)  “扑克蒙”蛋白又称“波克曼”蛋白

hz-2157P Polycystin 1(Polycystic Kidney Disease 1)  多囊肾蛋白1抗原

hz-2158P Polycystin 2(polycystic kidney disease 2)  多囊肾蛋白2抗体

hz-0876P phospho-AKT1/PKB1/2/3(pThr473)peptide  磷酸化蛋白激酶B抗原（丝氨酸磷酸化位点：473）

hz-1436P RKIP(Raf kinase inhibitor protein)  Raf激酶抑制蛋白抗原

hz-2146P pre-X protein[Hepatitis B virus]N-Terminus  乙肝病毒pre-X蛋白抗原（N端）

hz-2147P pre-X protein(CT)[Hepatitis B virus C-Terminus]  乙肝病毒pre-X蛋白抗原（C端）

hz-0532P PHD3 (Egln3; HIF-prolyl hydroxylase 3)  脯氨酸羟化酶(多肽)

hz-0433P ADAMTS-7(a disintegrin-like and metalloprotease-7 )  新型金属基质蛋白酶(抗原)

hz-0471P Adiponectin  脂联素（多肽抗原）

hz-0610P Adipo R1(adiponectin receptor 1)  脂联素受体-1(抗原)

hz-0611P Adipo R2(adiponectin receptor 2)  脂联素受体-2(抗原)

hz-1879P PSAP/PAP peptide  前列腺酸性磷酸酶抗原

hz-2241P PSAP/PAP peptide (human)  前列腺酸性磷酸酶抗原(人)

hz-0561P P-selectin  P选择素抗原

hz-1523P CD63/MLA1  溶酶体膜糖蛋白抗原

hz-0686P PTEN/MMAC1(CT)human, mo, rat, pig, dog, bovine, horse, chicken  一种肿瘤抑制基因抗原（C端）

hz-1299P Parvalbumin  微白蛋白/细小清蛋白抗原

hz-2265P Peptide YY  酪酪肽多肽（1-36）

hz-1221P Rad51  Rad51抗原