指状蛋白RET抗体

英文名称    RET    

中文名称    指状蛋白RET抗体    

别    名    C ret; CDHF 12; CDHF12; ELKS Fusion gene; HSCR 1; Hydroxyaryl protein kinase; MTC 1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET51; RET_HUMAN.    

 指状蛋白RET抗体       

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  细胞生物  免疫学  信号转导  生长因子和激素  激酶和磷酸酶  细胞膜受体      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    122kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human RET    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

 指状蛋白RET抗体产品介绍    background:
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq].

Function:
Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Act as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration.

Subunit:
Phosphorylated form interacts with the PBT domain of DOK2, DOK4 and DOK5. The phosphorylated form interacts with PLCG1 and GRB7 (By similarity). Interacts (not phosphorylated) with CC PTK2/FAK1 (via FERM domain). Extracellular cell-membrane anchored RET cadherin fragments form complex in neurons with reduced trophic status, preferentially at the contact sites between somas. Interacts with AIP in the pituitary gland; this interaction prevents the formation of the AIP-survivin complex. Binds to ARTN.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.

Post-translational modifications:
Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062. 
Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.

DISEASE:
Defects in RET may be a cause of colorectal cancer (CRC) [MIM:114500]. 
Defects in RET are a cause of Hirschsprung disease type 1 (HSCR1) [MIM:142623]. HSCR1 is a disorder of neural crest development characterized by the absence of intramural ganglion cells in the myenteric and submucosal plexuses of the gastrointestinal tract, often resulting in intestinal obstruction. Total colonic aganglionosis and total intestinal Hirschsprung disease also occur. Occasionally, MEN2A or FMTC occur in association with HSCR1. 
Defects in RET are the cause of medullary thyroid carcinoma (MTC) [MIM:155240]. MTC is a rare tumor derived from the C cells of the thyroid. Three hereditary forms are known, that are transmitted in an autosomal dominant fashion: (a) multiple neoplasia type 2A (MEN2A), (b) multiple neoplasia type IIB (MEN2B) and (c) familial MTC (FMTC), which occurs in 25-30% of MTC cases and where MTC is the only clinical manifestation.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. 
Contains 1 cadherin domain. 
Contains 1 protein kinase domain.

Gene ID:
6010

Database links:

Entrez Gene: 6010 Human

Entrez Gene: 212541 Mouse

Entrez Gene: 24717 Rat

Omim: 180380 Human

SwissProt: P08100 Human

SwissProt: P15409 Mouse

SwissProt: P51489 Rat

Unigene: 247565 Human

Unigene: 2965 Mouse

Unigene: 406156 Mouse

Unigene: 92530 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

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hz-0345M-hzy7 Mouse Anti-human IgM/Cy7  Cy7标记的小鼠抗人IgM

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hz-0294M-HRP Mouse Anti-Goat IgG/HRP  辣根过氧化物酶标记的小鼠抗羊IgG

hz-0294M-RBIThz Mouse Anti-Goat IgG/RBITC  罗丹明标记的小鼠抗羊IgG

hz-0294M-Bio Mouse Anti-Goat IgG/Bio  生物素标记的小鼠抗羊IgG

hz-0294M-Gold Mouse Anti-Goat IgG/Gold  胶体金标记的小鼠抗羊IgG

hz-0294M-hzy3 Mouse Anti-Goat IgG/Cy3  Cy3标记的小鼠抗羊IgG

hz-0294M-hzy5 Mouse Anti-Goat IgG/Cy5  Cy5标记的小鼠抗羊IgG

hz-0294M-hzy5.5 Mouse Anti-Goat IgG/Cy5.5  Cy5.5标记的小鼠抗羊IgG

hz-0294M-hzy7 Mouse Anti-Goat IgG/Cy7  Cy7标记的小鼠抗羊IgG

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hz-0294M-APhz Mouse Anti-Goat IgG/APC  APC标记的小鼠抗羊IgG

hz-0294M-AF350 Mouse Anti-Goat IgG/Alexa Fluor 350  Alexa Fluor 350标记的小鼠抗羊IgG

hz-0294M-AF488 Mouse Anti-Goat IgG/Alexa Fluor 488  Alexa Fluor 488标记的小鼠抗羊IgG

hz-0294M-AF555 Mouse Anti-Goat IgG/Alexa Fluor 555  Alexa Fluor 555标记的小鼠抗羊IgG

hz-0294M-AF647 Mouse Anti-Goat IgG/Alexa Fluor 647  Alexa Fluor 647标记的小鼠抗羊IgG

hz-0294M-PE-hzy5.5 Mouse Anti-Goat IgG/PE-Cy5.5  PE-Cy5.5标记的小鼠抗羊IgG

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