大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒

大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒中有三个必要的试剂：免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分：

(1)已包被抗原或抗体的固相载体(免疫吸附剂);

(2)酶标记的抗原或抗体(结合物);

(3)酶的底物;

(4)阴性对照品和阳性对照品(定性测定中)，参考标准品和控制血清(定量测定中);

(5)酶联物(结合物)及标本的稀释液;

(6) 大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒洗涤液;

(7)酶反应终止液。

酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物，可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法，这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基，大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。

大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒，仅供应包被用抗原或抗体，检测人员需自行包被。以下简述固相载体和包被过程。

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