大鼠穿孔蛋白1(PRF1)检测试剂盒

大鼠穿孔蛋白1(PRF1)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠穿孔蛋白1(PRF1)检测试剂盒中有三个必要的试剂：免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分：

(1)已包被抗原或抗体的固相载体(免疫吸附剂);

(2)酶标记的抗原或抗体(结合物);

(3)酶的底物;

(4)阴性对照品和阳性对照品(定性测定中)，参考标准品和控制血清(定量测定中);

(5)酶联物(结合物)及标本的稀释液;

(6) 大鼠穿孔蛋白1(PRF1)检测试剂盒洗涤液;

(7)酶反应终止液。

酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物，可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法，这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基，大鼠穿孔蛋白1(PRF1)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。

大鼠穿孔蛋白1(PRF1)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒，仅供应包被用抗原或抗体，检测人员需自行包被。以下简述固相载体和包被过程。

HZ-EL-RB1135c 兔线粒体肌酸激酶1A(CKMT1A)检测试剂盒   Rabbit CKMT1A (Creatine Kinase, Mitochondrial 1A ) ELISA Kit 

HZ-EL-RB1136c 兔碳酸酐酶XII(CA12)检测试剂盒 Rabbit CA12 (Carbonic Anhydrase XII) ELISA Kit

HZ-EL-RB1137c 兔抗角蛋白抗体(AKA)检测试剂盒 Rabbit AKA (Anti-Keratin Antibody) ELISA Kit

HZ-EL-RB1138c 兔血管紧张素I(ANG I)检测试剂盒 Rabbit ANG I (Angiotensin I) ELISA Kit

HZ-EL-RB1140c 兔组织蛋白酶B(CTSB)检测试剂盒 Rabbit CTSB (Cathepsin B) ELISA Kit

HZ-EL-RB1141c 兔周期素依赖性激酶5(CDK5)检测试剂盒 Rabbit CDK5 (Cyclin Dependent Kinase 5) ELISA Kit

HZ-EL-RB1142c 兔白介素8(IL-8)检测试剂盒 Rabbit IL-8 (Interleukin 8) ELISA Kit

HZ-EL-RB1143c 兔可溶性半乳糖凝集素3结合蛋白(LGALS3BP)检测试剂盒 Rabbit LGALS3BP (Lectin Galactoside Binding, Soluble 3 Binding Protein) ELISA Kit

HZ-EL-RB1144c 兔白细胞分化抗原CD97(CD97)检测试剂盒 Rabbit CD97 (Cluster of Differentiation 97) ELISA Kit 

HZ-EL-RB1145c 兔热休克蛋白27(HSP-27/HSPB1)检测试剂盒 Rabbit HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit

HZ-EL-RB1147c 兔丙酮酸脱氢酶β(PDHβ)检测试剂盒 Rabbit PDHβ (Pyruvate Dehydrogenase Beta) ELISA Kit 

HZ-EL-RB1148c 兔15脂加氧酶(15-LO)检测试剂盒 Rabbit 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

HZ-EL-RB1149c 兔肌球蛋白轻链1(MYL1)检测试剂盒 Rabbit MYL1 (Myosin Light Chain 1, Alkali, Fast Skeletal) ELISA Kit

HZ-EL-RB1150c 兔间隙连接蛋白26(CX26)检测试剂盒 Rabbit CX26 (Connexin 26) ELISA Kit 

HZ-EL-RB1151c 兔水通道蛋白3(AQP-3)检测试剂盒 Rabbit AQP-3 (Aquaporin 3) ELISA Kit 

HZ-EL-RB1152c 兔血管性血友病因子(VWF)检测试剂盒 Rabbit VWF (Von Willebrand Factor) ELISA Kit

HZ-EL-RB1153c 兔胎盘核糖核酸抑制剂(PRI)检测试剂盒 Rabbit PRI (Placental Ribonuclease Inhibitor) ELISA Kit

HZ-EL-RB1154c 兔白细胞分化抗原CD109(CD109)检测试剂盒 Rabbit CD109 (Cluster of Differentiation 109) ELISA Kit 

HZ-EL-RB1155c 兔粘蛋白5B(MUC5B)检测试剂盒 Rabbit MUC5B (Mucin 5 Subtype B) ELISA Kit

HZ-EL-RB1157c 兔补体C3转化酶(C3c)检测试剂盒 Rabbit C3c (Complement C3 Convertase) ELISA Kit

HZ-EL-RB1158c 兔原钙黏素1(PCDH1)检测试剂盒 Rabbit PCDH1 (Protocadherin 1) ELISA Kit

HZ-EL-RB1160c 兔GATA结合蛋白2(GATA2)检测试剂盒 Rabbit GATA2 (GATA Binding Protein 2) ELISA Kit 

HZ-EL-RB1161c 兔白介素2受体β(IL-2Rβ)检测试剂盒 Rabbit IL-2Rβ (Interleukin 2 Receptor Beta) ELISA Kit

HZ-EL-RB1162c 兔肌腱蛋白C(TNC)检测试剂盒 Rabbit TNC (Tenascin C) ELISA Kit

HZ-EL-RB1165c 兔多巴胺受体D5(D5R/DRD5)检测试剂盒 Rabbit D5R/DRD5 (Dopamine Receptor D5) ELISA Kit 

HZ-EL-RB1166c 兔跨膜丝氨酸蛋白酶2(TMPRSS2)检测试剂盒 Rabbit TMPRSS2 (Transmembrane Protease, Serine 2) ELISA Kit 

HZ-EL-RB1167c 兔轻肽神经丝蛋白(NEFL)检测试剂盒 Rabbit NEFL (Neurofilament, Light Polypeptide) ELISA Kit

HZ-EL-RB1169c 兔软骨糖蛋白39(GP39)检测试剂盒 Rabbit GP39 (Glycoprotein 39, Cartilage) ELISA Kit

HZ-EL-RB1170c 兔乳腺癌抗雌激素药物耐药性基因1(BCAR1)检测试剂盒 Rabbit BCAR1 (Breast Cancer Anti Estrogen Resistance 1) ELISA Kit

HZ-EL-RB1171c 兔雌二醇(E2)检测试剂盒 Rabbit E2 (Estradiol) ELISA Kit

HZ-EL-RB1172c 兔TATA框结合蛋白相关因子1(TAF1)检测试剂盒 Rabbit TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit 

HZ-EL-RB1173c 兔白介素15(IL-15)检测试剂盒 Rabbit IL-15 (Interleukin 15) ELISA Kit