大鼠生长调节致癌基因β(GROβ)检测试剂盒

大鼠生长调节致癌基因β(GROβ)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠生长调节致癌基因β(GROβ)检测试剂盒中有三个必要的试剂：免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分：

(1)已包被抗原或抗体的固相载体(免疫吸附剂);

(2)酶标记的抗原或抗体(结合物);

(3)酶的底物;

(4)阴性对照品和阳性对照品(定性测定中)，参考标准品和控制血清(定量测定中);

(5)酶联物(结合物)及标本的稀释液;

(6) 大鼠生长调节致癌基因β(GROβ)检测试剂盒洗涤液;

(7)酶反应终止液。

酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物，可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法，这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基，大鼠生长调节致癌基因β(GROβ)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。

大鼠生长调节致癌基因β(GROβ)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒，仅供应包被用抗原或抗体，检测人员需自行包被。以下简述固相载体和包被过程。

HZ-EL-RB0809c 兔核因子κB受体激活因子配基(RANκL)检测试剂盒 Rabbit RANκL (Receptor Activator of  Nuclear Factor Kappa B Ligand) ELISA Kit

HZ-EL-RB0810c 兔α1抗胰蛋白酶(α1-AT)检测试剂盒 Rabbit α1-AT (Alpha 1-Antitrypsin) ELISA Kit

HZ-EL-RB0811c 兔黑色素瘤标记物(MART/Melan-A)检测试剂盒 Rabbit MART/Melan-A (Melanoma Marker A) ELISA Kit

HZ-EL-RB0812c 兔促胰液素(SCT)检测试剂盒 Rabbit SCT (Secretin) ELISA Kit

HZ-EL-RB0813c 兔紧密结合蛋白1(TJP1)检测试剂盒 Rabbit TJP1 (Tight Junction Protein 1) ELISA Kit 

HZ-EL-RB0814c 兔迟现抗原(VLA)检测试剂盒 Rabbit VLA (Very Late Appearing Antigen) ELISA Kit

HZ-EL-RB0815c 兔白介素1受体拮抗剂(IL-1ra)检测试剂盒 Rabbit IL-1ra (Interleukin 1 Receptor Antagonist) ELISA Kit

HZ-EL-RB0816c 兔尿嘧啶核苷磷酸化酶2(UPP2)检测试剂盒 Rabbit UPP2 (Uridine Phosphorylase 2) ELISA Kit

HZ-EL-RB0817c 兔叉头框蛋白C1(FOXC1)检测试剂盒 Rabbit FOXC1 (Forkhead Box Protein C1) ELISA Kit

HZ-EL-RB0818c 兔胆固醇酯转移蛋白(CETP)检测试剂盒 Rabbit CETP (Cholesteryl Ester Transfer Protein) ELISA Kit

HZ-EL-RB0820c 兔细胞周期素D1(CCND1)检测试剂盒 Rabbit CCND1 (Cyclin-D1) ELISA Kit

HZ-EL-RB0821c 兔黏膜地址素细胞黏附分子(MAdCAM1)检测试剂盒 Rabbit MAdCAM1 (Mucosal Addressin Cell Adhesion Molecule 1) ELISA Kit

HZ-EL-RB0822c 兔脂肪酸合酶(FASN)检测试剂盒 Rabbit FASN (Fatty Acid Synthase) ELISA Kit

HZ-EL-RB0823c 兔胃泌素抑制肽(GIP)检测试剂盒   Rabbit GIP (Gastric Inhibitory Polypeptide) ELISA Kit

HZ-EL-RB0824c 兔蛋白激酶Bγ(PKBG)检测试剂盒 Rabbit PKBG (Protein Kinase B Gamma) ELISA Kit 

HZ-EL-RB0825c 兔组织多肽抗原(TPA)检测试剂盒 Rabbit TPA (Tissue Polypeptide Antigen) ELISA Kit

HZ-EL-RB0826c 兔皮肤反应因子/炎性因子(SRF/IF)检测试剂盒 Rabbit SRF/IF (Skin Reactive Factor/Inflammatory Factor) ELISA Kit

HZ-EL-RB0828c 兔L1细胞粘附分子(L1CAM/CD171)检测试剂盒 Rabbit L1CAM/CD171 (L1-Cell Adhesion Molecule) ELISA Kit

HZ-EL-RB0829c 兔血红蛋白C(HbC)检测试剂盒   Rabbit HbC (Hemoglobin C) ELISA Kit

HZ-EL-RB0830c 兔Ⅲ型前胶原羧基端原肽(PⅢCP)检测试剂盒 Rabbit PⅢCP (Procollagen Ⅲ C-Terminal ProPeptide) ELISA Kit 

HZ-EL-RB0831c 兔丝氨酸蛋白酶(PRSS)检测试剂盒 Rabbit PRSS (Serine Protease) ELISA Kit

HZ-EL-RB0832c 兔促黄体生成激素(LH)检测试剂盒 Rabbit LH (Luteinizing Hormone) ELISA Kit

HZ-EL-RB0833c 兔CCAAT增强子结合蛋白δ(C/EBPδ)检测试剂盒 Rabbit C/EBPδ (CCAAT/Enhancer Binding Protein, Delta) ELISA Kit

HZ-EL-RB0834c 兔肿瘤坏死因子α转化酶(TACE)检测试剂盒 Rabbit TACE (TNF α Converting Enzyme) ELISA Kit

HZ-EL-RB0835c 兔封闭蛋白1(CLDN1)检测试剂盒 Rabbit CLDN1 (Claudin 1) ELISA Kit 

HZ-EL-RB0836c 兔纤溶抑制因子(TAFI)检测试剂盒 Rabbit TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit