大鼠低密度脂蛋白免疫复合物(LDL-IC)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠低密度脂蛋白免疫复合物(LDL-IC)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠低密度脂蛋白免疫复合物(LDL-IC)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠低密度脂蛋白免疫复合物(LDL-IC)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠低密度脂蛋白免疫复合物(LDL-IC)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK1716c 猴甲状腺素抗体(TAb)检测试剂盒 Monkey TAB (Thyroxine Antibody) ELISA Kit
HZ-EL-MK1717c 猴蛋白二硫化物异构酶A3(PDIA3)检测试剂盒 Monkey PDIA3 (Protein Disulfide Isomerase A3) ELISA Kit
HZ-EL-MK1718c 猴凝血因子XIII A1多肽(F13A1)检测试剂盒 Monkey F13A1 (Coagulation Factor XIII A1 Polypeptide) ELISA Kit
HZ-EL-MK1719c 猴巨噬细胞炎性蛋白5(MIP-5)检测试剂盒 Monkey MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit
HZ-EL-MK1720c 猴甲肟前列腺素D2(PGD2-MOX)检测试剂盒 Monkey PGD2-MOX (Prostaglandin D2-Methoxime) ELISA Kit
HZ-EL-MK1721c 猴肌动蛋白束蛋白(FSCN)检测试剂盒 Monkey FSCN (Fascin) ELISA Kit
HZ-EL-MK1722c 猴X型胶原(COL10)检测试剂盒 Monkey COL10 (Collagen Type X) ELISA Kit
HZ-EL-MK1723c 猴丝裂原激活蛋白激酶激酶1(MAP2K1)检测试剂盒 Monkey MAP2K1 (Mitogen Activated Protein Kinase Kinase 1) ELISA Kit
HZ-EL-MK1724c 猴嗜中性粒细胞防御素α1(DEFα1)检测试剂盒 Monkey DEFα1 (Defensin Alpha 1, Neutrophil) ELISA Kit
HZ-EL-MK1725c 猴肿瘤蛋白P53(TP53)检测试剂盒 Monkey TP53 (Tumor Protein p53) ELISA Kit
HZ-EL-MK1726c 猴43KDa Tar DNA结合蛋白(TDP43)检测试剂盒 Monkey TDP43 (Tar DNA Binding Protein 43KDa )ELISA Kit
HZ-EL-MK1727c 猴谷胱甘肽S-转移酶α1(GSTα1)检测试剂盒 Monkey GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit
HZ-EL-MK1728c 猴III型前胶原(PCIII)检测试剂盒 Monkey PCIII (Procollagen III) ELISA Kit
HZ-EL-MK1729c 猴低密度脂蛋白受体相关蛋白6(LRP6)检测试剂盒 Monkey LRP6 (Low Density Lipoprotein Receptor Related Protein) ELISA Kit
HZ-EL-MK1730c 猴烟酰胺腺嘌呤二核苷酸磷酸氧化酶1(NOX1)检测试剂盒 Monkey NOX1 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1) ELISA Kit
HZ-EL-MK1731c 猴激活素A(ACVA)检测试剂盒 Monkey ACVA (Activin A) ELISA Kit
HZ-EL-MK1732c 猴线粒体超氧化物歧化酶2(SOD2)检测试剂盒 Monkey SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit
HZ-EL-MK1733c 猴补体因子8b(C8b)检测试剂盒 Monkey C8b (Complement Component 8b) ELISA Kit
HZ-EL-MK1734c 猴粒细胞巨噬细胞集落刺激因子抗体(GM-CSF Ab)检测试剂盒 Monkey GM-CSF Ab (Anti-Granulocyte Macrophage Colony Stimulating Factor Antibody) ELISA Kit
HZ-EL-MK1735c 猴淋巴细胞功能相关抗原2(LFA-2/CD2)检测试剂盒 Monkey LFA-2/CD2 (Lymphocyte Function Associated Antigen 2) ELISA Kit
HZ-EL-MK1736c 猴骨粘连蛋白(ON)检测试剂盒 Monkey ON (Osteonectin) ELISA Kit
HZ-EL-MK1737c 猴α乳清蛋白(α-La)检测试剂盒 Monkey α-La (Alpha-Lactalbumin) ELISA Kit
HZ-EL-MK1739c 猴可溶性细胞因子受体(sCKR)检测试剂盒 Monkey sCKR (Soluble Cytokine Receptor) ELISA Kit
HZ-EL-MK1740c 猴Slit同源物2(Slit2)检测试剂盒 Monkey Slit2 (Slit Homolog 2) ELISA Kit
HZ-EL-MK1741c 猴凝血因子XI(F11)检测试剂盒 Monkey F11 (Coagulation Factor XI) ELISA Kit
HZ-EL-MK1742c 猴载脂蛋白B(ApoB)检测试剂盒 Monkey ApoB (Apolipoprotein B) ELISA Kit
HZ-EL-MK1743c 猴肝配蛋白A4 (EFNA4)检测试剂盒 Monkey EFNA4 (Ephrin A4) ELISA Kit
HZ-EL-MK1744c 猴趋化素样因子超家族成员6(CKLFSF6)检测试剂盒 Monkey CKLFSF6 (Chemokine Like Factor Superfamily 6) ELISA Kit
HZ-EL-MK1746c 猴脂肪细胞型脂肪酸结合蛋白(FABP4)检测试剂盒 Monkey FABP4 (Fatty Acid-binding Protein 4, Adipocyte) ELISA Kit