人癌胚铁蛋白(CEF)ELISA试剂盒试验所需自备物品:
1. 酶标仪(450nm波长滤光片)
2. 高精度移液器,EP管及一次性吸头:0.5-10μl, 2-20μl, 20-200μl, 200-1000μl
3. 37℃恒温箱, 双蒸水或去离子水
4. 吸水纸
检测前准备工作:
1. 请提前20分钟从冰箱中取出人癌胚铁蛋白(CEF)ELISA试剂盒,平衡至室温。
2. 将浓缩洗涤液用双蒸水稀释(1:25)。未用完的放回4℃。从冰箱中取出的浓缩洗涤液可能有结晶,属于正常现象,可用40℃水浴微加热使结晶完全溶解后再配制洗涤液。(加热温度不要超过50℃)使用时洗涤液应为室温。
3. 标准品: 加入标准品&标本稀释液1.0ml至冻干标准品中,静置10分钟,待其充分溶解后,轻轻混匀(浓度为5000 pg/ml)。然后根据需要进行倍比稀释(注:不要直接在板中进行倍比稀释)。建议配制成以下浓度: 2500、1250、625、312.5、156.2、78.1、39.05、0 pg/ml。样品稀释液直接作为空白孔 0 pg/ml。如配制2500pg/ml标准品:取0.5ml (不要少于0.5ml )5000pg/ml的上述标准品加入含有0.5ml样品稀释液的EP管中,混匀即可,其余浓度以此类推。人癌胚铁蛋白(CEF)ELISA试剂盒
4. 生物素化抗体工作液:实验前计算当次实验所需用量(以100μl/孔计),实际配制时应多配制100-200μl。使用前15分钟,以生物素化抗体稀释液稀释浓缩生物素化抗体(1:100)成工作浓度。当日使用。
5. 酶结合物工作液:实验前计算当次实验所需用量(以100μl/孔计),实际配制时应多配制 100-200μl。使用前15分钟,以生物素化抗体稀释液稀释浓缩生物素化抗体(1:100)成工作浓度。当日使用。
人癌胚铁蛋白(CEF)ELISA试剂盒其他相关试剂盒:
Caprine (Goat)
SEA052Cp 羊胰岛素样生长因子结合蛋白1(IGFBP1)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Capra hircus; Caprine (Goat)
SEA129Cp 羊组织金属蛋白酶抑制因子3(TIMP3)ELISA试剂盒 ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Capra hircus; Caprine (Goat)
SEA214Cp 羊窖蛋白(CAV1)ELISA试剂盒 ELISA Kit for Caveolin 1 (CAV1) Capra hircus; Caprine (Goat)
SEB949Cp 羊转化生长因子β3(TGFβ3)ELISA试剂盒 ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) Capra hircus; Caprine (Goat)
SEA133Cp 羊肿瘤坏死因子α(TNFα)ELISA试剂盒 ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) Capra hircus; Caprine (Goat)
SEA054Cp 羊胰岛素样生长因子结合蛋白3(IGFBP3)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Capra hircus; Caprine (Goat)
SEA055Cp 羊胰岛素样生长因子结合蛋白4(IGFBP4)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4) Capra hircus; Caprine (Goat)
SEA056Cp 羊白介素10(IL10)ELISA试剂盒 ELISA Kit for Interleukin 10 (IL10) Capra hircus; Caprine (Goat)
SEA058Cp 羊白介素12B(IL12B)ELISA试剂盒 ELISA Kit for Interleukin 12B (IL12B) Capra hircus; Caprine (Goat)
SEA059Cp 羊白介素12A(IL12A)ELISA试剂盒 ELISA Kit for Interleukin 12A (IL12A) Capra hircus; Caprine (Goat)
SEA060Cp 羊白介素13(IL13)ELISA试剂盒 ELISA Kit for Interleukin 13 (IL13) Capra hircus; Caprine (Goat)
SEA061Cp 羊白介素15(IL15)ELISA试剂盒 ELISA Kit for Interleukin 15 (IL15) Capra hircus; Caprine (Goat)
SEA218Cp 羊转化生长因子β2(TGFβ2)ELISA试剂盒 ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) Capra hircus; Caprine (Goat)
SEA064Cp 羊白介素18(IL18)ELISA试剂盒 ELISA Kit for Interleukin 18 (IL18) Capra hircus; Caprine (Goat)
SEA178Cp 羊α2-HS糖蛋白(αHSG)ELISA试剂盒 ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG) Capra hircus; Caprine (Goat)
SEA172Cp 羊血小板因子4(PF4)ELISA试剂盒 ELISA Kit for Platelet Factor 4 (PF4) Capra hircus; Caprine (Goat)
SEA071Cp 羊白介素1α(IL1α)ELISA试剂盒 ELISA Kit for Interleukin 1 Alpha (IL1a) Capra hircus; Caprine (Goat)