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产品编号:FS1381
产品名称:酸性肉汤
产品规格:250g/瓶
用途:用于酸性罐头食品无菌检验
酸性肉汤使用方法:
(1)称取Pfizer 肠球菌选择性琼脂(PSE 琼脂)50g,加入蒸馏水或去离子水1 L,搅拌加热煮沸至完全溶解,分装三角瓶,121℃高压灭菌20min,备用。
(2)检样的稀释处理。
(3)选取3个合适的稀释级的样品1 mL,分别注入平皿,每个稀释级做2—3个平板,每皿加15—20 mL已冷至45℃左右的YPD培养基,加毕立即摇匀,待凝固后倒置于20—25℃培养箱中培养72h。
(4)观察结果。对于菌落微小点计困难的平板可延续培养到4—5d再行观察计数。
(5)计数:通常选择菌落数在30-100之间的稀释级的平板计数,取其平均数乘以稀释倍数报告。
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