MICA抗体-MICA抗体说明书

Rabbit Anti-MICA抗体说明书 antibody

FROM:ABCAM

Catalog Number :RS-1610R

Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:

The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-Anti-MICA抗体说明书 antibodytumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Function:

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Specificity :

–Anti-MICA is a rabbit polyclonal antibody

–specific for MICA of rat

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 43 kDa

Application : Anti-MICA抗体说明书 antibody

ELISA=1:500-1000

IHC-P=1:100-500

IHC-F=1:100-500

IF=1:150-200

– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.