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凋亡相关蛋白激酶1抗体

供货周期：一周

品牌： Abcam

规格： 0.2ml/200μg

货号：

CAS号：

报价： ¥1

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产品介绍

凋亡相关蛋白激酶1抗体英文名称 Anti-DAPK1

中文名称凋亡相关蛋白激酶1抗体

别名 DAP Kinase 1; DAPK1; DAK1; DAP K1; DAPK 1; DAPK; DAPK1; Death Associated Protein Kinase 1; Death Associated Protein Kinase 1; Death-associated protein kinase 1; DKFZp781I035; DAPK1_HUMAN.

公司凋亡相关蛋白激酶1抗体应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。

浓度 1mg/1ml

规格 0.1ml/100μg 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat

产品类型一抗

研究领域肿瘤细胞生物信号转导细胞凋亡激酶和磷酸酶

蛋白分子量 predicted molecular weight: 160kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human DAPK1 C-terminus

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

凋亡相关蛋白激酶1抗体产品介绍 DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.

Function : Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.

Subunit : Interacts with KLHL20. Interacts (via death domain) with MAPK1 and MAPK3. Interacts with MAP1B (via N-terminus). Interacts (via death domain) with UNC5B (via death domain). Interacts with PRKD1 in an oxidative stress-regulated manner. Interacts with PIN1, PDCD6, BECN1, GRINB, TSC2 and STX1A. Interacts (via kinase domain) with DAPK3 (via kinase domain).

Subcellular Location : Cytoplasm. Colocalizes with the actin filament system.

Tissue Specificity : Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Post-translational modifications : Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.

Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

DAP kinase subfamily.

Contains 10 ANK repeats.

Contains 1 death domain.

Contains 1 protein kinase domain.

Database links :

【存储要求】连续使用时4°C存储，保质期六个月；凋亡相关蛋白激酶1抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：

1）WB：Western Blotting 免疫印迹

2）IH：Immunohistochemistry 免疫组化

3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)

4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)

5）IC：Immunocytochemistry 免疫细胞化学