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酸化D酪氨酸激酶衰减蛋白1抗体

供货周期：一周

品牌： Abcam

规格： 0.1ml/100μg

货号：

CAS号：

报价： ¥1

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产品介绍

酸化D酪氨酸激酶衰减蛋白1抗体英文名称 Anti-phospho-DOK1(Tyr362)

中文名称磷酸化D酪氨酸激酶衰减蛋白1抗体

别名 DOK1 (phospho Y362); DOK1 (phospho Tyr362); p-DOK1 (phospho Y362); docking protein 1 (downstream of tyrosine kinase 1); Docking protein 1 (p62(dok)) (Downstream of tyrosine kinase 1) (pp62).; docking protein 1 62kD (downstream of tyrosine kinase 1); Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK-1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62; pp62.

公司酸化D酪氨酸激酶衰减蛋白1抗体应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。

浓度 1mg/1ml

规格 0.1ml/100μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow

产品类型一抗磷酸化抗体

研究领域肿瘤心血管信号转导细胞凋亡 G蛋白偶联受体

蛋白分子量 predicted molecular weight: 62kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated Synthesised phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr362

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

酸化D酪氨酸激酶衰减蛋白1抗体产品介绍 Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Function : DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

Subunit : Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.

Subcellular Location : Isoform 1: Cytoplasm. Isoform 3: Cytoplasm, perinuclear region.

Tissue Specificity : Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.

Post-translational modifications : Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated by LYN.

Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.

Isoform 3 contains a N-acetylmethionine at position 1.

Similarity : Belongs to the DOK family. Type A subfamily.

Contains 1 IRS-type PTB domain.

Contains 1 PH domain.

Database links : UniProtKB/Swiss-Prot: Q99704.1

【存储要求】连续使用时4°C存储，保质期六个月；酸化D酪氨酸激酶衰减蛋白1抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：

1）WB：Western Blotting 免疫印迹

2）IH：Immunohistochemistry 免疫组化

3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)

4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)

5）IC：Immunocytochemistry 免疫细胞化学