别 名 ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP; ATP binding cassette sub family G (WHITE) member 2; ATP binding cassette transporter G2; ATP-binding cassette sub-family G member 2; BCRP1; BMDP; Breast cancer resistance protein; CD338; CDw338; CDw338 antigen; EST157481; GOUT1; MGC102821; Mitoxantrone resistance associated protein; Mitoxantrone resistance-associated protein; MRX; Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2; MXR; MXR1; Placenta specific ATP binding cassette transporter; Placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; UAQTL1.
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 免疫学 神经生物学 干细胞 转运蛋白 细胞表面分子
蛋白分子量 predicted molecular weight: 72kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ABCG2
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
三磷酸腺苷结合转运蛋白G超家族成员2抗体产品介绍 An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.
Function : Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Subunit : Monomer or homodimer; disulfide-linked.
Subcellular Location : Cell membrane; Multi-pass membrane protein.
Tissue Specificity : Highly expressed in placenta. Low expression in small intestine, liver and colon.
Similarity : Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
Database links : UniProtKB/Swiss-Prot: Q9UNQ0.3
Hematopoietic/Neural Stem Cell Marker （造血/神经干细胞标志物)
纯度：在实验的任何阶段，确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1～l0ug/带)。
定量：如果抗体还不纯，有一个快捷的定量方法，即通过SDS-PAGE电泳分离出轻、重链，然后和已知的标准染色带比较。如果需要分析许多样本，用免疫测定法对抗体定量较容易。如果抗体是经过纯化的，可通过测蛋白总量代替上述两种方法，有一简单的方法，即紫外吸收法。三磷酸腺苷结合转运蛋白G超家族成员2抗体的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。
抗原结合活性：一般说来，纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而，如果最终抗体产物的作用不如原来所预料的好，检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性，以标定每一步中的总抗体量，这将有助于较好的估计通过纯化所丢失的活性。
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