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当前位置: 环亚生物 > 最新动态 > 如何进行长片段基因组DNA质控?—Pippin Pulse脉冲场电泳系统

如何进行长片段基因组DNA质控?—Pippin Pulse脉冲场电泳系统

环亚生物

2023/09/28 12:34

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随着三代测序技术的发展和普及,全基因组测序技术也取得了长足发展。基因组DNA文库的制备质量直接影响测序结果,因而基因组DNA的质控越来越受到重视。而实验室常规的琼脂糖凝胶电泳只能分离20kb以下的DNA分子,当DNA分子大于20kb时,就无法实现有效分离。

针对超长DNA片段的分离和质控,美国Sage Science公司拥有超高性价比的Pippin Pulse脉冲场电泳系统。Pippin Pulse使用简单,无专用耗材,自带预设程序,能很好的电泳分离0.5Kb到430Kb的基因组DNA片段。Pippin Pulse具有一个简单的软件界面,自带6种预设程序,可以直接分离多种不同大小范围DNA长片段,此外,软件还支持自定义模式,用户可以自编程分离不同范围的DNA片段。Pippin Pulse包括电源、电泳槽、操控仪器的电脑和用户手册,无特殊耗材,使用成本低,经济耐用。

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Pippin Pulse脉冲场电泳系统

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Pippin Pulse脉冲场电泳系统预设程序

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Pippin Pulse脉冲场电泳系统结果图示

Pippin Pulse脉冲场电泳系统应用案例(部分展示)

1. Pippin Pulse 与PacBio三代测序仪联用,控制文库片段分布,充分发挥长片段测序优势

  • Takeuchi, Tadashi, et al. "Fatty acid overproduction by gut commensal microbiota exacerbates obesity." Cell Metabolism (2023).

  • Hadzhiev, Yavor, et al. "The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation." Developmental Cell 58.2 (2023): 155-170.

  • Kurokochi, Hiroyuki, et al. "Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake)." DNA Research 30.3 (2023): dsad006.

  • Hixson, Kim K., et al. "Annotated genome sequence of a fast-growing diploid clone of red alder (Alnus rubra Bong.)." G3: Genes, Genomes, Genetics (2023): jkad060.

  • Field, Matt A., et al. "The Australian dingo is an early offshoot of modern breed dogs." Science Advances (2022): eabm5944.

  • Pootakham, Wirulda, et al. "A Chromosome-Scale Genome Assembly of Mitragyna speciosa (Kratom) and the Assessment of Its Genetic Diversity in Thailand." Biology 11.10 (2022): 1492.

  • Rahman, M. Julhasur, et al. "LINE-1 retrotransposons facilitate horizontal gene transfer into poxviruses." Elife 11 (2022): e63327.

  • Zhao, Qing, et al. "The reference genome sequence of Scutellaria baicalensis provides insights into the evolution of wogonin biosynthesis." Molecular plant 12.7 (2019): 935-950.

2. Pippin Pulse 与 Nanopore三代测序仪联用,质控更长、更高质量的DNA,进一步提升测序长度

  • Long, Meng, et al. "Comparative genomic analysis provides insights into taxonomy and temperature adaption of Aeromonas salmonicida." Journal of Fish Diseases 46.5 (2023): 545-561.

  • Keraite, Ieva, et al. "A method for multiplexed full-length single-molecule sequencing of the human mitochondrial genome." Nature Communications 13.1 (2022): 5902.

  • Ong, Chian Teng, et al. "Adaptive sampling during sequencing reveals the origins of the bovine reproductive tract microbiome across reproductive stages and sexes." Scientific reports 12.1 (2022): 15075.

  • Wunderlich, Stephanie, et al. "Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies." Molecular Therapy-Methods & Clinical Development 26 (2022): 84-94.

  • Russo, Alessia, et al. "Low-input high-molecular-weight DNA extraction for long-read sequencing from plants of diverse families." Frontiers in Plant Science 13 (2022): 1494.

  • Player, Robert A., et al. "A novel Canis lupus familiaris reference genome improves variant resolution for use in breed-specific GWAS." Life science alliance 4.4 (2021).

  • Young, Amy E., et al. "Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull." Nature biotechnology 38.2 (2020): 225-232.

  • Belser, Caroline, et al. "Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps." Nature plants 4.11 (2018): 879-887.

3. Pippin Pulse 与 Bionano分子光谱仪联用,确保HMW DNA,保证分析结果准确性

  • Secomandi, Simona, et al. "A chromosome-level reference genome and pangenome for barn swallow population genomics." Cell Reports 42.1 (2023): 111992.

  • Dahn, Hollis A., et al. "Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing." GigaScience 11 (2022).

  • Palmada-Flores M, Orkin J D, Haase B, et al. A high-quality, long-read genome assembly of the endangered ring-tailed lemur (Lemur catta)[J]. GigaScience, 2022, 11.

  • Canaguier A, Guilbaud R, Denis E, et al. Oxford Nanopore and Bionano Genomics technologies evaluation for plant structural variation detection[J]. BMC genomics, 2022, 23(1): 1-17.

  • Shukla H, Suryamohan K, Khan A, et al. Near-chromosomal de novo assembly of Bengal tiger genome reveals genetic hallmarks of apex-predation[J]. bioRxiv, 2022.

  • Rhie, Arang, et al. "Towards complete and error-free genome assemblies of all vertebrate species." Nature 592.7856 (2021): 737-746.

  • Belser, Caroline, et al. “Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing.” Communications biology 4.1 (2021): 1047.

  • Zheng, Yuting, et al. "Rapid prenatal diagnosis of Facioscapulohumeral Muscular Dystrophy 1 by combined Bionano optical mapping and karyomapping." Prenatal Diagnosis 40.3 (2020): 317-323.

环亚生物科技有限公司作为美国Sage Science公司Pippin Pulse脉冲场电泳系统在中国区的独家代理商,自2011年以来将Sage Science的产品线引入国内,一直为国内用户提供专业的全自动核酸片段回收系统的安装测试、应用培训,技术支持与售后维护工作,赢得客户的一致好评与信任。环亚生物科技有限公司将一如既往地支持越来越多的Sage Science用户。


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