甲酰胺杂交缓冲液Hybit2

杂交缓冲液是一种先进的甲酰胺混合液能够有效提高微阵列芯片的杂交效果，在与cDNA和长链寡核苷酸反应中，通过增加信号强度，并减少背景提高微阵列杂交反应。
该甲酰胺混合液可降低杂交温度。

杂交缓冲液HybIt2 通过加快溶液中的互补目标序列与微阵列表面的和标记的探针分子之间的碱基配对，提高了微阵列杂交反应的有效性和明确性。
? Increases hybridization signals by accelerating reaction kinetics.
? Reduces surface tension enabling a uniform hybridization layer.
? Compatible with many different surface chemistries.
? Arrives as a pre-mixed 1.25X solution that is sterile and ready to use.
? Costs <€1 dollar per hybridization reaction. 1- Labelling and Purifying the hybrization probe using a suitable purification method.
2- Resuspend the probe in 1.0 μl of pure H20 and add 4.0 μl of Hybridization Solution.
3- Hybridize the probe to the microarray. The hybridization reaction must be done at 42°C.
4- Wash away the unbound probe.(Wash Buffers recommended).
5- San the slides and quantify the hybridization result