核糖核酸酶III(RNASEN)试剂盒抗原修复方法:
常用抗原修复液:柠檬酸缓冲液(0.01M pH6.0)、EDTA 抗原修复液(pH8.0 或9.0)等等。
核糖核酸酶III(RNASEN)试剂盒酶消化修复法酶消化修复
切片脱蜡水化处理,TBS 冲洗,在组织上滴加胃蛋白酶或胰蛋白酶,37℃孵育20~30min 后 TBS 冲洗即可。
核糖核酸酶III(RNASEN)试剂盒微波抗原修复法:
微波盒中加入抗原修复液微波加热至沸腾,将脱蜡水化后的切片置于耐高温塑料切片架上,放入已沸腾的缓冲液中,中档或高档继续微波 10~15min,取出微波盒冷却至室温后,自来水冲洗,取出切片。因不同微波炉微波处理时间存在差异,须自行调整。
核糖核酸酶III(RNASEN)试剂盒的详细资料:
中文名称:核糖核酸酶III(RNASEN)试剂盒
规格:48孔/96孔
保存:2-8℃
方法:elisa
货期:现货
检测方法:酶联免疫法/酶免法(ELISA)
主要作用:科研
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