2012/09/13 15:29
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细胞培养-细胞休眠
It is an environmental cell adaptation characterized by… the cell cycle slowing down, with cells in G1 & Mitosis passing through slowly to be arrested and gathered in the G2 phase………
2012/09/13
高氧环境下细胞培养的细胞损伤情况分析!
传统的培养方式往往使得组织/细胞处于高氧环境,易产生自由式的ROS,从而导致DNA突变或者断裂、转录错误、蛋白合成受到影响等等,使得研究人员的实验数据产生偏差,如:研究细胞凋亡等
2012/09/13
细胞培养-细胞收获
Disadvantages of the scrapers Upon observing the scraping edge under a microscope, it also reveals additional disadvantages... The scraper edges are large and rounded relative to the cell size and therefore, does not allows a real wiping action such as a snow plough, but acts more like a road roller crushing under the scraper blade the first line of cells .
2012/09/13
细胞培养结果比较
Problems that come from culturing cells (mainly stem cells) in physiologically irrelevant O2 levels There are genes which are normally expressed in physiologic conditions in tissue cells, that in cell culture (21% O2) become suppressed and disappear or are significantly reduced
2012/09/13