2012/09/13 15:22
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细胞培养-细胞分选
Special Petakas called “Easy Transfer” (Petaka ET) are manufactured with a set of paramagnetic micro-tools inside. These micro-tools (“surfers”), powered by the magnetic field created by a specific device (the Flipper), glide over the cells without scraping or shearing, lifting them intact using strictly “gentle physical forces” created by media vortex. Using Easy Transfer Petakas, ensures a clean cell release and suspension, avoiding cell damage from enzymes and surface molecules losses.
2012/09/13
细胞培养-细胞休眠
It is an environmental cell adaptation characterized by… the cell cycle slowing down, with cells in G1 & Mitosis passing through slowly to be arrested and gathered in the G2 phase………
2012/09/13
细胞培养-细胞收获
Disadvantages of the scrapers Upon observing the scraping edge under a microscope, it also reveals additional disadvantages... The scraper edges are large and rounded relative to the cell size and therefore, does not allows a real wiping action such as a snow plough, but acts more like a road roller crushing under the scraper blade the first line of cells .
2012/09/13
细胞培养结果比较
Problems that come from culturing cells (mainly stem cells) in physiologically irrelevant O2 levels There are genes which are normally expressed in physiologic conditions in tissue cells, that in cell culture (21% O2) become suppressed and disappear or are significantly reduced
2012/09/13