重组小鼠肝素结合性表皮生长因子/Mouse HB-EGF
Proheparin-Binding EGF-like Growth Factor Mouse Recombinant
产品详情:
CATALOGUE NUMBER
CYT-068
SYNONYMS
Heparin-binding EGF-like growth factor, DTR, HEGFL, diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), DTSF, proheparin-binding epidermal growth factor-like growth factor.
INTRODUCTION
HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF’s ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.
DESCRIPTION
HB-EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids (63-148 a.a.) and having a molecular mass of 9.8 kDa.
The HB-EGF is purified by proprietary chromatographic techniques
SOURCE
Escherichia Coli.
PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freeze-dried) powder.
FORMULATION
The protein was filtered (0.2μm) and lyophilized from a concentrated solution containing 10mM PB and 500mM NaCl, pH7.4.
SOLUBILITY
It is recommended to reconstitute the lyophilized Mouse HB-EGF in sterile 18M-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized Mouse HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
AMINO ACID SEQUENCE
DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL.
BIOLOGICAL ACTIVITY
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 units/mg.
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.