Recombinant Human MutL Homolog-1(MLH1)

MLH1 Human
MutL Homolog-1 Human Recombinant
Catalog No. PRO-526

Synonyms:
DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1, COCA2, FCC2, HNPCC, hMLH1, HNPCC2, MGC5172.

Introduction:
MLH1 is an 87kDa protein involved in mismatched nucleotides during DNA replication or repair process. Mutations in the human MLH1 (hMLH1) and hMLH2 genes contribute to the development of sporadic colorectal carcinoma and some other both hereditary and sporadic forms of human cancer.
MLH1 is identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic modifications in microsatellite sequences (RER+ phenotype) found in HNPCC.

Description:
MLH1 Human Recombinant (a.a. 557-756) expressed in E.coli, MLH1 is antibody reactive. The MLH1 is fused to a GST tag and purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
The MLH1 at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.

Stability:
Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
Please prevent freeze-thaw cycles.

Applications:
• ELISA
• Inhibition Assays
• Western Blotting

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.