Recombinant LALF

LALF
Limulus Antilipopolysaccharide Factor Recombinant
Catalog No. PRO-361

Synonyms:
Anti-lipopolysaccharide factor, anti-LPS, LALF.

Introduction:
LALF has been isolated from the membranes of amebocytes of Limulus polyphemus. LALF is a single-chain polypeptide with a molecular weight of 11,800. The principal structure of the molecule consists of 102 amino acids and is partially homologous with structures of molecules in the lactalbumin-lysozyme family.
LALF has a high affinity binding for LPS and bactericidal for gram negative bacteria. LALF binds the lipid A part of a variety of chemically diverse LPSs.
It can be used for endotoxin removal and detection. LALF reacts with specific antisera in ELISA and Western blots.

Description:
Recombinant LALF, entire coding region for limulus antilipopolysaccharide factor derived from Pichia pastoris. LALF is a 12,000 dalton protein.
The LALF is purified by proprietary chromatographic techniques.

Source:
Pichia pastoris

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Solubility:
It is recommended to reconstitute the lyophilized LALF in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized LALF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LALF should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
Antibacterial activity against E. coli less than 10ug/ml at 10e6 PFU/ml. Binds to and inactivates endotoxin (LPS).

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.