核心参数
产地类别: 进口
单分子检测水平的定量细胞生物学检测仪器。
受激发射损耗(STED)是一种强大的显微技术,可以观察到空间分辨率低于衍射极限的荧光结构。Alba-STED使用脉冲激发和脉冲耗尽方法(pSTED)结合数字频域荧光寿命成像(FastFLIM)来记录时间分辨光子,从而提高图像分辨率并分离具有相同激发波长的两个荧光标记物。
关键特色 : Alba-STED for FLIM/FFS:
• pSTED (Pulsed excitation and pulsed STED)
• FastFLIM for time-resolved pSTED acquisition
• Improved image resolution using the phasor plot
• Dual-label excitation
• Fast image acquisition (dwell time: 0.2 μs)
• High dynamic range (signal up to 60 million counts/s)
测试实例展示 Alba-STED for FLIM/FFS
Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells, acquired by FastFLIM.
Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells, acquired by FastFLIM.
Confocal images of 60nm fluorescence beads (left); pSTED images (middle); sharpening the pSTED image using a binary filter based on the phasor plots (right).
Dual labels can be separated using pSTED and FastFLIM. Atto 647N and Atto 655 were used as labels; they both are excited by the 640 nm laser. The two dyes are first separated using the phasor plots, and then assigned with two different false colors (Atto 647N - yellow, Atto 655 - purple) to produce the processed and merged pSTED image of the two labels.
参考论文:
Monomeric cohesin state revealed by live-cell single-molecule spectroscopy.
Liu, W., Biton, E., Pathania, A., Matityahu, A., Irudayaraj, J., Onn, I.
EMBO Rep. 2019 Dec 29:e48211. doi: 10.15252/embr.201948211. [Epub ahead of print]
Photon-separation to enhance the spatial resolution of pulsed STED microscopy.
Tortarolo, G., Sun, Y., Teng, K.W., Ishitsuka, Y., Lanzanó, L., Selvin, P.R., Barbieri, B., Diaspro, A., Vicidomini, G.
Nanoscale. 2019 Jan 9. doi: 10.1039/c8nr07485b. [Epub ahead of print]
A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses.
Wang, R., Brustlein, S., Mailfert, S., Fabre, R., Fallet, M., Sivankutty, S., Rigneault, H., Marguet, D.
Methods. 2018 May 1;140-141:212-222. doi: 10.1016/j.ymeth.2018.02.010. Epub 2018 Feb 14.
A Novel Pulsed STED Microscopy Method Using FastFLIM and the Phasor Plots
Sun, Y.,Tortarolo G., Teng, K.-W., Ishitsuka, Y., Coskun, U.C., Liao, S.-C., Diaspro, A., Vicidomini, G., Selvin, P.R., Barbieri, B.
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691C (February 21, 2017)
ISS共聚焦显微镜Alba-STED的工作原理介绍
共聚焦显微镜Alba-STED的使用方法?
ISSAlba-STED多少钱一台?
共聚焦显微镜Alba-STED可以检测什么?
共聚焦显微镜Alba-STED使用的注意事项?
ISSAlba-STED的说明书有吗?
ISS共聚焦显微镜Alba-STED的操作规程有吗?
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ISSAlba-STED有现货吗?
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企业名称
天津东方科捷科技有限公司
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信用代码
120113000107875
成立日期
2011-03-02
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550
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仪器仪表、机械设备技术开发、咨询、转让、服务;实验室仪器、光电设备、控制设备、机械设备零配件制造;机械加工;仪器仪表、机械设备、教学设备、电子元件批发兼零售;通信设备、家用电器零售;从事国家法律法规允许经营的进出口业务。(以上经营范围涉及行业许可的凭许可证件,在有效期限内经营,国家有专项专营规定的按规定办理)
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