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重组人表皮生长因子/EGF Human
产品编号：CYT-217
产品规格：100μg/500μg/1mg
产品规格：100μg/500μg/1mg
| Catalogue Number | CYT-217 |
| Synonyms | Urogastrone, URG, EGF. |
| Introduction | Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. |
| Description |
Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The EGF is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity |
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR. |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg. |
| Protein content |
EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. |
| References |
1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas. Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full 2.Title:Evaluation of bioactivity and effect of polymeric stabilizers during heat treatment for the unfolded fraction of human epidermal growth factor. Publication:SEN'I GAKKAISHI vol.67,nov.8(2011) Link:http://www.jstage.jst.go.jp/article/fiber/67/8/185/_pdf 3.Title:GPR56 is essential for testis development and male fertility in mice. Publication:Article first published online: 27 OCT 2010 DOI: 10.1002/dvdy.22468 Link:http://onlinelibrary.wiley.com/doi/10.1002/dvdy.22468/full 4.Title:Changing the Receptor Specificity of Anthrax Toxin. Publication:doi: 10.1128/?mBio.00088-12 1 May 2012 mBio vol. 3 no. 3 e00088-12 Link:http://mbio.asm.org/content/3/3/e00088-12.full 5.Title:characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152 Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027152 |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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人表皮生长因子(EGF)ELISA试剂盒使用说明书
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医疗/卫生
2016/03/24
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重组人表皮生长因子/EGF Human由厦门慧嘉生物科技有限公司为您提供，货号CYT-217，规格：100μg/500μg/1mg，CAS号：，如您想了解更多关于重组人表皮生长因子/EGF Human价格、重组人表皮生长因子/EGF Human结构式、批发、用途等信息，欢迎咨询。除供应重组人表皮生长因子/EGF Human外，还可为您提供人唾液酸(SA)ELISA试剂盒Human,SA ELISA Kit、人维生素D3(VD3)ELISA试剂盒Human VD3 ELISA kit、人胎盘催乳素(HPL)ELISA试剂盒Human HPL ELISA试剂盒等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，厦门慧嘉科技客户服务电话，售前、售后均可联系。
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