LALF
Limulus Antilipopolysaccharide Factor Recombinant
Catalog No. PRO-361
Synonyms:
Anti-lipopolysaccharide factor, anti-LPS, LALF.
Introduction:
LALF has been isolated from the membranes of amebocytes of Limulus polyphemus. LALF is a single-chain polypeptide with a molecular weight of 11,800. The principal structure of the molecule consists of 102 amino acids and is partially homologous with structures of molecules in the lactalbumin-lysozyme family.
LALF has a high affinity binding for LPS and bactericidal for gram negative bacteria. LALF binds the lipid A part of a variety of chemically diverse LPSs.
It can be used for endotoxin removal and detection. LALF reacts with specific antisera in ELISA and Western blots.
Description:
Recombinant LALF, entire coding region for limulus antilipopolysaccharide factor derived from Pichia pastoris. LALF is a 12,000 dalton protein.
The LALF is purified by proprietary chromatographic techniques.
Source:
Pichia pastoris
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Solubility:
It is recommended to reconstitute the lyophilized LALF in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized LALF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LALF should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
Antibacterial activity against E. coli less than 10ug/ml at 10e6 PFU/ml. Binds to and inactivates endotoxin (LPS).
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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上海市松江区陆家圈路168弄113号
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